{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T19:15:51Z","timestamp":1791573351625,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T00:00:00Z","timestamp":1767657600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Ischemic stroke is a prevalent neurological disorder often resulting in brain tissue injury and blood\u2013brain barrier (BBB) damage. Luteolin (LUT), a natural polyphenolic compound in grape skin, wine, and other plants, has neuroprotective properties against ischemic stroke. However, it remains unclear whether its protective effect involves alterations in BBB integrity. This study aimed to examine the BBB\u2010protective impact of LUT in a photothrombotic (PT) mouse model and oxygen\u2013glucose deprivation (OGD) cell model and investigate its underlying mechanism. First, we used neurofunctional scoring, 2,3,5\u2010triphenyltetrazolium chloride staining, Nissl staining, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to evaluate the neuroprotective effects of LUT in ischemic stroke. Furthermore, we assessed the impact of LUT on BBB permeability using Evans Blue staining, brain water content, transendothelial electrical resistance, FITC, and flow cytometry techniques. We also examined the expression of tight junction (TJ) proteins related to BBB integrity using Western blotting. Finally, we validated the potential mechanism of action of LUT using short hairpin RNA (shRNA) technology. LUT alleviated the cerebral infarct area and neuronal apoptosis in the PT mouse model, reduced brain water content and Evans Blue leakage, decreased bEnd.3 cell apoptosis in the OGD model, increased transmembrane resistance, and reduced FITC leakage. LUT also counteracted the degradation of TJ proteins and decreased the expression of MMP9. Furthermore, the improvement was weakened when LRP1 was interfered with using shRNA. Our study suggests that LUT has the potential to improve BBB damage and exert neuroprotective effects following ischemic stroke. The mechanism of action may involve the inhibition of LRP1 and subsequent reduction in MMP9 expression.<\/jats:p>","DOI":"10.1002\/ptr.70121","type":"journal-article","created":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T14:43:23Z","timestamp":1767710603000},"page":"4898-4912","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Luteolin Improves\n                    <scp>BBB<\/scp>\n                    Integrity Following Ischemic Stroke Injury by Downregulating the Expression of\n                    <scp>LRP1<\/scp>\n                    and\n                    <scp>MMP9<\/scp>"],"prefix":"10.1002","volume":"40","author":[{"given":"Jia\u2010min","family":"Li","sequence":"first","affiliation":[{"name":"Precision Pharmacy and Drug Development Center, Department of Pharmacy, Tangdu Hospital Air Force Medical University  Xi'an China"},{"name":"Key Laboratory of Pharmacodynamic Mechanism and Material Basis of Traditional Chinese Medicine, Shaanxi Provincial Administration of Traditional Chinese Medicine Shaanxi University of Chinese Medicine  Xianyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao\u2010yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Precision Pharmacy and Drug Development Center, Department of Pharmacy, Tangdu Hospital Air Force Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Sun","sequence":"additional","affiliation":[{"name":"Precision Pharmacy and Drug Development Center, Department of Pharmacy, Tangdu Hospital Air Force Medical University  Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming\u2010lei","family":"Fan","sequence":"additional","affiliation":[{"name":"Precision Pharmacy and Drug Development Center, Department of Pharmacy, Tangdu Hospital Air Force Medical University  Xi'an 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