{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T01:59:46Z","timestamp":1789523986387,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T00:00:00Z","timestamp":1763424000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100015377","name":"Shanghai Key Laboratory of Molecular Imaging","doi-asserted-by":"publisher","award":["18DZ2260400"],"award-info":[{"award-number":["18DZ2260400"]}],"id":[{"id":"10.13039\/501100015377","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Curcumin, a highly hydrophobic phenolic compound, has demonstrated potent anticancer activity. However, poor water solubility and a short biological half\u2010life hinder its clinical application. We developed Curcumin\u2010loaded Pluronic F\u2010127 nanoparticles to enhance the bioavailability of Curcumin. It has been reported that excessive autophagy is considered to be the main mechanism of death of various cancer cells. The aim of this study is to investigate whether the Curcumin\u2010loaded Pluronic F\u2010127 nanoparticles exert anti\u2010colon cancer effects by modulating autophagy and explore the specific regulatory mechanism. The proliferative, migratory and apoptotic activity of CT26 cells was assessed by CCK\u20108 assay, wound healing assay and flow cytometry, respectively. In addition, levels of reactive oxygen species (ROS) were examined by DCFH\u2010DA assay in\u00a0vitro. ATP levels were detected by the ELISA. The expression levels of autophagy\u2010related proteins and apoptosis\u2010related proteins were visualized by western blotting. Curcumin\u2010loaded Pluronic F\u2010127 nanoparticle inhibited the proliferation of CT26 cells and the growth of colon tumours by promoting cellular autophagy. In addition, Curcumin\u2010loaded Pluronic F\u2010127 nanoparticle reduced mitochondrial membrane potential and impaired mitochondrial function by increasing ROS levels, disrupting ATP biosynthesis. Then the decreased ATP\/AMP ratio activated the AMPK\/mTOR\/ULK1 autophagy signalling pathway and induced autophagy\u2010dependent apoptosis in CT26 cells. Intriguingly, the apoptosis and excessive autophagy induced by Curcumin\u2010loaded Pluronic F\u2010127 nanoparticle were reversed after pretreatment with the antioxidant N\u2010acetyl\u2010L\u2010cysteine. Curcumin\u2010loaded Pluronic F\u2010127 nanoparticle induced autophagy\u2010dependent apoptosis through ROS\u2010AMPK\/mTOR\/ULK1 signalling in colon cancer cells. Our study offers new insights for colon cancer treatment advancement.<\/jats:p>","DOI":"10.1002\/ptr.70122","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T22:25:48Z","timestamp":1763504748000},"page":"176-193","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Curcumin Nanoparticle Induces Apoptosis in Colon Cancer Cells via\n                    <scp>ROS<\/scp>\n                    \u2010Mediated\n                    <scp>AMPK<\/scp>\n                    \/\n                    <scp>mTOR<\/scp>\n                    \/\n                    <scp>ULK1<\/scp>\n                    Autophagy Pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Bingqing","family":"Liang","sequence":"first","affiliation":[{"name":"Department of Scientific Research Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"},{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"},{"name":"Shanghai Key Laboratory of Molecular lmaging Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengxian","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Scientific Research Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"},{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"},{"name":"Shanghai Key Laboratory of Molecular lmaging Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Cardiology Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxiao","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Scientific Research Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"},{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"},{"name":"Shanghai Key Laboratory of Molecular lmaging Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anhong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"},{"name":"Shanghai Key Laboratory of Molecular lmaging Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"},{"name":"Department of Scientific Research Shanghai University of Traditional Chinese Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihua","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongping","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Cardiology Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengfeng","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzhong","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Scientific Research Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"},{"name":"Department of Scientific Research Xinxiang Medical College  Xinxiang Henan China"},{"name":"Shanghai Key Laboratory of Molecular lmaging Zhoupu Hospital, Shanghai University of Medicine and Health Sciences  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2016.02.027"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.06159\u201011"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13183"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2019.12.004"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010019\u20101234\u20108"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596\u2010021\u20102469\u20100"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2014.150"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26190"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.2217\/nnm\u20102021\u20100229"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2697"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2019103111"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/21691401.2023.2238756"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2018.12.128"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12102936"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2020.1841953"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/emm.2016.81"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2022.113092"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2007.03.005"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0168\u20103659(02)00009\u20103"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2021.111438"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201403799"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.Can\u201006\u20104257"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.06.043"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419\u2010019\u20101728\u20105"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010020\u20101138\u20104"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2018.1522467"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nano.2011.06.011"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41418\u2010023\u201001178\u20101"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392\u2010019\u20100087\u20100"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2023.127429"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2006.09.025"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885\u2010020\u201007256\u20108"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.02.010"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21565"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.05.023"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2018.1505153"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12143"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2013.5371"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072364"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.6b07031"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.01.008"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27020445"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2018.04.050"},{"issue":"4","key":"e_1_2_12_45_1","first-page":"905","article-title":"Involvement of Bcl\u20102 Family Members, Phosphatidylinositol 3\u2032\u2010Kinase\/AKT and Mitochondrial p53 in Curcumin (Diferulolylmethane)\u2010Induced Apoptosis in Prostate Cancer","volume":"30","author":"Shankar S.","year":"2007","journal-title":"International Journal of Oncology"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2021.5279"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20102006\u2010957450"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcis.2019.12.096"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells11091479"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/1759\u20107714.13904"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20051033"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204\u2010022\u201003421\u2010z"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2015.02.116"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046\u2010019\u20101424\u20104"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.45176"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20109966\u201030\u201087"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.13085fp"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.2147\/ijn.S154054"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.06.040"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2007.03.010"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.2c04407"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2021.188569"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7303"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70122","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.70122","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70122","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:19:23Z","timestamp":1768285163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.70122"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,18]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1002\/ptr.70122"],"URL":"https:\/\/doi.org\/10.1002\/ptr.70122","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,18]]},"assertion":[{"value":"2024-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}