{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:43:43Z","timestamp":1793727823058,"version":"4.6.1"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chronic alcoholic encephalopathy (CAE) is a condition induced by alcohol consumption, with a huge demand for research on its prevention and treatment drugs. Puerarin, the principal active compound found in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pueraria lobata<\/jats:italic>\n                    <\/jats:styled-content>\n                    , has been traditionally utilized in ethnopharmacology to mitigate alcoholic brain injury and rectify imbalances in intestinal flora. The study was aimed to investigate the mechanism by which puerarin exerts its anti\u2010CAE effect. The CAE mice model induced by alcohol were treated with oral administration of puerarin. First, the effects of puerarin on cognitive function, motor ability, and hippocampal tissue pathology along with the expression of TLR4, Myd88, NF\u2010\u03baB, IL\u20101\u03b2, and IL\u20106 of brain and fecal LPS were investigated. Finally, the composition of the gut microbiome of fecal and TJs (Claudin\u20101 and Occludin) in the intestine and colon, focusing on the production and transporter of LPS, were measured. The findings revealed that puerarin administration significantly ameliorated motor deficits, anxiety\u2010like behaviors, and cognitive impairments in CAE mice. Histopathological analysis revealed puerarin reduced hippocampal damage and decreased Iba1 immunoreactivity, indicating attenuated neuroinflammation. Puerarin treatment downregulated the protein expression of IL\u20101\u03b2, IL\u20106, TLR4, Myd88, and NF\u2010\u03baB in brain. Notably, puerarin restored intestinal barrier integrity by upregulating Claudin\u20101 and Occludin expression. Intestinal flora analysis demonstrated that puerarin treatment increased the abundance of beneficial bacteria (e.g.,\n                    <jats:italic>norank_f_Eubacterium_coprostanoligenes_group<\/jats:italic>\n                    ) while reducing pathogenic bacteria (e.g.,\n                    <jats:italic>Escherichia\u2010Shigella<\/jats:italic>\n                    ). The study showed that puerarin exerts a treatment CAE effect, which may be related to modulation of the \u201cmicrobiota\u2010gut\u2010brain axis\u201d LPS\/TLR4\/NF\u2010\u03baB inflammatory pathway.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70197","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T11:08:47Z","timestamp":1768475327000},"page":"1003-1016","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effect of Puerarin on Chronic Alcoholic Encephalopathy by Modulating the \u201cMicrobiota\u2010Gut\u2010Brain Axis\u201d Lipopolysaccharides\/Toll\u2010Like Receptors 4\/Nuclear Factor Kappa\u2010B Inflammatory Pathway"],"prefix":"10.1002","volume":"40","author":[{"given":"Lei","family":"Zhang","sequence":"first","affiliation":[{"name":"Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1918-8854","authenticated-orcid":false,"given":"Bo","family":"Li","sequence":"additional","affiliation":[{"name":"Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals Zhejiang University of Technology  Hangzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa","family":"Ye","sequence":"additional","affiliation":[{"name":"Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University  Hangzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9131-3018","authenticated-orcid":false,"given":"Lin Zi","family":"Li","sequence":"additional","affiliation":[{"name":"Jingmen Central Hospital  Jingmen Hubei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5219-3882","authenticated-orcid":false,"given":"Shan","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences &amp; Institute of Materia Medica Shandong First Medical University &amp; Shandong Academy of Medical Sciences, State Key Laboratory of Advanced Drug Delivery and Release Systems, Key Laboratory for Biotechnology Drugs of National Health Commission (Shandong Academy of Medical Sciences), Key Lab for Rare &amp; Uncommon Diseases of Shandong Province  Jinan People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5324-3984","authenticated-orcid":false,"given":"Shan Shan","family":"Lei","sequence":"additional","affiliation":[{"name":"Department of Medicine Zhejiang Academy of Traditional Chinese Medicine  Hangzhou Zhejiang People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,15]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1037\/pha0000463"},{"key":"e_1_2_12_3_1","first-page":"71","article-title":"Establishment of Chronic Alcoholic Brain Injury Model in Mice","volume":"33","author":"Cai Y.","year":"2023","journal-title":"Chinese Journal of Comparative Medicine"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S254369"},{"key":"e_1_2_12_5_1","first-page":"53","article-title":"Research Progress on Chemical Constituents and Pharmacological Effects of Gegen","volume":"12","author":"Chen Y.","year":"2021","journal-title":"Pharmacyand Clinics of Chinese Materia Medica"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.35946\/arcr.v44.1.04"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-013-1233-3"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/adb.13018"},{"key":"e_1_2_12_9_1","first-page":"152","article-title":"Study on the Antidotal Effect of Kudzu Glycoside Oral Administration on Acute Alcohol Poisoning","volume":"2","author":"Fan Z. 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