{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T20:57:25Z","timestamp":1791838645392,"version":"4.3.4"},"reference-count":64,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T00:00:00Z","timestamp":1768262400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diabetic liver injury (DLI) is a chronic complication of the liver caused by diabetes mellitus, and its pathomechanism has not been fully elucidated. Punicalagin (PU), a polyphenol extracted from pomegranate peel, has physiological activities such as anti\u2010inflammatory. In this study, the effects of PU on DLI and its molecular mechanisms were investigated. In\u00a0vitro and in\u00a0vivo studies were conducted using streptozotocin\u2010induced diabetic mouse models and high glucose\u2010induced HepG2 cells. After PU intervention, the effects of PU on DLI were assessed by histopathology, immunohistochemistry, western blot, immunofluorescence and transmission electron microscopy. The results showed that PU improved the pathological damage of liver tissue in diabetic mice, reduced the levels of inflammatory factors such as TNF\u2010\u03b1, IL\u201018 and IL\u20101\u03b2 in serum and liver, down\u2010regulated the protein levels of NEK7, NLRP3 and Caspase1 in liver and HepG2 cells, and attenuated the fluorescence co\u2010localization of NEK7 and NLRP3 in HepG2 cells. Additionally, PU up\u2010regulated the expression of mitochondrial fusion\u2010related proteins OPA1 and Mfn2 and their transfer to mitochondria, and inhibited the expression of mitochondrial fission\u2010related proteins Drp1 and p\u2010Drp1 (Ser616). The mitochondrial fusion inhibitor MYLS22 reversed the inhibitory effect of PU on NEK7\u2010NLRP3 complex. In conclusion, the present study shows that PU inhibits NEK7\u2010NLRP3 complex activation by regulating mitochondrial dynamics, thereby reducing liver inflammation and alleviating DLI.<\/jats:p>","DOI":"10.1002\/ptr.70208","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T07:47:32Z","timestamp":1768376852000},"page":"986-1002","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["New Insights of Punicalagin in Alleviating Diabetic Liver Injury: Inhibition of\n                    <scp>NEK7<\/scp>\n                    \u2010\n                    <scp>NLRP3<\/scp>\n                    via Modulating Mitochondrial Dynamics"],"prefix":"10.1002","volume":"40","author":[{"given":"Xiuying","family":"Tan","sequence":"first","affiliation":[{"name":"Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rou","family":"Zhang","sequence":"additional","affiliation":[{"name":"Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"You","sequence":"additional","affiliation":[{"name":"Xiangya School of Public Health Central South University  Changsha China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Yang","sequence":"additional","affiliation":[{"name":"Xiangya School of Public Health Central South University  Changsha 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