{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:24:15Z","timestamp":1789503855790,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972040"],"award-info":[{"award-number":["81972040"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fibroblast\u2010like synoviocytes (FLS) drive rheumatoid arthritis (RA) progression. Bacopaside I (BSI), a major component of the anti\u2010RA herb\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bacopa monnieri<\/jats:italic>\n                    <\/jats:styled-content>\n                    , demonstrates anti\u2010arthritic effects in RA animal models; however, its precise anti\u2010rheumatic mechanisms, especially regarding suppression of RA\u2010FLS pathogenicity, remain unclear. Collagen\u2010induced arthritis (CIA) rats and TNF\u2010\u03b1\u2010stimulated RA\u2010FLS were used as in\u00a0vivo and in\u00a0vitro models of RA. We studied BSI's therapeutic potential in CIA rats and its influences on TNF\u2010\u03b1\u2010induced migration, invasion, and inflammation in RA\u2010FLS, focusing on the underlying mechanism of Wnt\/\u03b2\u2010catenin pathway inhibition. BSI exhibited arthritis\u2010alleviating activity in CIA rats, as evidenced by reductions in paw swelling, arthritis index, and histological damage to ankle joints. It also decreased serum and synovial levels of IL\u20101\u03b2, IL\u20106, and TNF\u2010\u03b1, indicating anti\u2010inflammatory effects in\u00a0vivo. At non\u2010cytotoxic concentrations, BSI inhibited migration, invasion, and F\u2010actin remodeling in TNF\u2010\u03b1\u2010stimulated RA\u2010FLS. Similar to its anti\u2010inflammatory activity in\u00a0vivo, BSI decreased pro\u2010inflammatory factor production in\u00a0vitro, including IL\u20101\u03b2, IL\u20106, IL\u20108, MMP\u20102, and MMP\u20109. Mechanistically, BSI treatment inhibited Wnt\/\u03b2\u2010catenin pathway activation in both CIA rat synovium and TNF\u2010\u03b1\u2010stimulated RA\u2010FLS, as demonstrated by decreased Wnt1, p\u2010GSK\u20103\u03b2 (Ser9), and \u03b2\u2010catenin protein levels, increased p\u2010\u03b2\u2010catenin, reduced \u03b2\u2010catenin nuclear translocation, and a lower TOP\/FOP ratio. Importantly, the critical involvement of this pathway was further confirmed by the loss of BSI's benefits following \u03b2\u2010catenin overexpression in TNF\u2010\u03b1\u2010stimulated RA\u2010FLS. BSI ameliorates arthritis severity and RA\u2010FLS pathogenicity by suppressing the Wnt\/\u03b2\u2010catenin pathway, highlighting its promise as a novel candidate for RA treatment.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70251","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T05:58:26Z","timestamp":1770098306000},"page":"1903-1918","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bacopaside I Ameliorates Collagen\u2010Induced Arthritis in Rats and the Pathogenic Behaviors of Fibroblast\u2010Like Synoviocytes via Wnt\/\u03b2\u2010Catenin Pathway Suppression"],"prefix":"10.1002","volume":"40","author":[{"given":"Ling\u2010ling","family":"Li","sequence":"first","affiliation":[{"name":"Inflammation and Immune Mediated Diseases Laboratory of Anhui Province, School of Pharmacy Anhui Medical University  Hefei Anhui 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