{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,11]],"date-time":"2026-11-11T00:22:48Z","timestamp":1794356568773,"version":"4.9.0"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T00:00:00Z","timestamp":1611360000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672143"],"award-info":[{"award-number":["81672143"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:sec><jats:label\/><jats:p>Glucocorticoid (GC) administration is one of the main causes of osteonecrosis of the femoral head (ONFH). Inflammation, especially the TLR4\/NF\u2010\u03baB pathway, has been demonstrated to play a pivotal role in the pathogenesis of GC\u2010induced ONFH. Calycosin, the main bioactive extract of <jats:italic>Astragali Radix<\/jats:italic>, could substantially regulate the TLR4\/NF\u2010\u03baB pathway. Therefore, in this study, we hypothesized that calycosin could exert beneficial effects in GC\u2010induced ONFH. In vitro, effects of calycosin on the osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs) were determined using Alizarin red staining, alkaline phosphatase activity examination, and osteogenic\u2010related gene assay. Meanwhile, inflammatory cytokines were detected by enzyme\u2010linked immunosorbent assay. In vivo, 60 male Sprague\u2013Dawley rats were randomly separated into three groups: the control group, the methylprednisolone (MPS) group, and the MPS\u2009+\u2009calycosin group. The results showed that calycosin could significantly promote dynamic bone formation and retard TLR4\/NF\u2010\u03baB pathway. in vivo investigations indicated that calycosin could decrease the morbidity of ONFH and alleviate pathological manifestations within the femoral head. Meanwhile, calycosin could protect osseous blood supply and facilitate dynamic bone formation. The findings collectively demonstrated that calycosin could ameliorate GC\u2010induced ONFH in rat and might become a potential candidate for pharmaceutical prevention of this intractable disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ptr.7028","type":"journal-article","created":{"date-parts":[[2021,1,23]],"date-time":"2021-01-23T09:48:45Z","timestamp":1611395325000},"page":"2824-2835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Calycosin modulates inflammation via suppressing <scp>TLR4<\/scp>\/<scp>NF\u2010\u03baB<\/scp> pathway and promotes bone formation to ameliorate glucocorticoid\u2010induced osteonecrosis of the femoral head in rat"],"prefix":"10.1002","volume":"35","author":[{"given":"Daoyu","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Orthopedic Surgery Shanghai Jiao Tong University Affiliated Sixth People's Hospital  Shanghai 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