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Additionally,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pueraria montana var. lobata<\/jats:italic>\n                    <\/jats:styled-content>\n                    is considered a functional food widely recognized to enhance the immune system and treat hyperlipidemia. We aim to evaluate the effects of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pueraria montana var. lobata<\/jats:italic>\n                    <\/jats:styled-content>\n                    extract (PLE) on the development of inflammation and the progression to metabolic dysfunction\u2010associated steatotic liver disease (MASLD) by investigating the effects and underlying mechanisms both in\u00a0vivo and in\u00a0vitro under metabolic stress. To address whether PLE can reverse established MASLD\/fibrosis, we performed a robust hepatosteatosis study in mice fed with a Western diet (WD) and PLE. AML12 and HepG2 cells were exposed to OA and\/or PA at different concentrations and combinations. Intracellular lipids and cell viability were detected with Oil Red O staining and CCK8 assay, respectively. Confocal microscopy was employed to analyze mitochondrial functions and their relative positions to peroxisomes. PLE treatment significantly reduced lipid accumulation, inflammatory response, liver injury, and fibrosis in mice fed a high\u2010fat diet. RNA\u2010sequencing indicated that the key mechanism underlying the anti\u2010MASLD effects of PLE was higher peroxisome activity and the subsequent increase in \u03b2\u2010oxidation flux. PLE concurrently decreased cholesterol\u2010raising saturated fatty acids and increased polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs) in AML12 mouse liver cells treated with OA. Confocal images revealed the intracellular transport route between mitochondria and peroxisomes after PLE treatment. There are currently no FDA\u2010approved therapies for MASLD. Our study demonstrates that PLE prevents metabolic stress\u2010induced MASLD progression through direct activation of CPT1A signaling, suggesting that PLE may be considered a therapeutic candidate for the treatment of MASLD.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70331","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T05:41:51Z","timestamp":1775626911000},"page":"3688-3703","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>\n                      <i>Pueraria montana var. lobata<\/i>\n                    <\/scp>\n                    Root Extract Alleviates Metabolic Dysfunction\u2010Associated Steatotic Liver Disease by Increasing Mitochondrial \u03b2\u2010Oxidation and Mitochondrial\u2010Peroxisome Contact"],"prefix":"10.1002","volume":"40","author":[{"given":"Han\u2010Lin","family":"Lu","sequence":"first","affiliation":[{"name":"Graduate Institute of Chinese Medicine, School of Chinese Medicine, China Medical University  Taichung Taiwan"},{"name":"Department of Chinese Medicine Tainan Sin\u2010Lau Hospital  Tainan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Der\u2010Yen","family":"Lee","sequence":"additional","affiliation":[{"name":"Graduate Institute of Integrated Medicine, China Medical University  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Wen","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Cancer Center for Traditional Chinese Medicine, Department of Medical Research China Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung\u2010Yu","family":"Chen","sequence":"additional","affiliation":[{"name":"Research Center for Cancer Biology China Medical University  Taichung 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