{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T04:26:32Z","timestamp":1791260792928,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T00:00:00Z","timestamp":1775952000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473029"],"award-info":[{"award-number":["32473029"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Triple\u2010negative breast cancer (TNBC) is aggressive with limited treatments. Although the natural flavonoid Apigenin (API) shows anti\u2010tumor potential, its mechanism in TNBC remains unclear. This study investigated API's role in inducing ferroptosis and inhibiting glycolysis to suppress TNBC. Molecular docking predicted API's binding to ferroptosis\u2010 and glycolysis\u2010related proteins. In\u00a0vitro, 4T1 and MDA\u2010MB\u2010231 TNBC cells were used to assess API's effects on viability, migration, invasion, and key metabolic markers (Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , MDA, ROS, GSH, lactic acid, glucose, ATP, OCR, ECAR), and protein expression (GPX4, SLC7A11, TFR, FPN1, FTH1, FTL, FSP1, PKM2, GLUT1, GLUT4, HK2, LDHA) using RT\u2010qPCR and Western blotting. In\u00a0vivo, a 4T1 tumor xenograft model evaluated API's impact on tumor growth, protein expression, and toxicity (H&amp;E staining). Molecular docking indicated good binding affinity of API with ferroptosis\u2010 and glycolysis\u2010related proteins. In\u00a0vitro, API inhibited the viability, migration, and invasion of TNBC cells. API significantly increased Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , MDA, and ROS levels while decreasing reduced GSH levels, downregulated GPX4, SLC7A11, FPN1, FTH1, FTL, and FSP1 expression, and upregulated TFR expression, indicating ferroptosis induction. API also decreased lactic acid, ATP, and ECAR levels while increasing intracellular glucose and OCR levels, downregulating PKM2, GLUT1, GLUT4, HK2, and LDHA expression, demonstrating glycolysis inhibition. In\u00a0vivo, API significantly inhibited tumor growth in the 4T1 xenograft model without obvious toxicity and regulated the expression of ferroptosis and glycolysis\u2010related proteins. These findings establish API as a promising natural anti\u2010TNBC drug by simultaneously targeting ferroptosis and aerobic glycolysis.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70337","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T05:35:33Z","timestamp":1776058533000},"page":"3741-3757","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Apigenin Inhibits Triple\u2010Negative Breast Cancer Growth by Dual Targeting\n                    <scp>GPX4<\/scp>\n                    \/\n                    <scp>SLC7A11<\/scp>\n                    \u2010Mediated Ferroptosis and\n                    <scp>PKM2<\/scp>\n                    \/\n                    <scp>GLUT1<\/scp>\n                    \u2010Mediated Aerobic Glycolysis"],"prefix":"10.1002","volume":"40","author":[{"given":"Mingmei","family":"Zhu","sequence":"first","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danping","family":"Peng","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun 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Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2427-0593","authenticated-orcid":false,"given":"Hongyue","family":"Xu","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4697-0208","authenticated-orcid":false,"given":"Baochun","family":"Lu","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Liao","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sijia","family":"Niu","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyan","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin University, Department of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine Jilin University, Center of Infectious Diseases and Pathogen Biology Jilin University  Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Cui","sequence":"additional","affiliation":[{"name":"The First Hospital of Jilin 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