{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:58:41Z","timestamp":1785855521228,"version":"3.56.0"},"reference-count":56,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Psoralea corylifolia<\/jats:italic>\n                    <\/jats:styled-content>\n                    Linn. exhibits osteogenic effects; however, the mechanism by which its active component Bakuchiol (BAK) alleviates glucocorticoid\u2010induced osteoporosis (GIOP) remains unclear. We aim to investigate BAK's therapeutic effects and potential mechanisms in GIOP. We treated GIOP mice with BAK. We evaluated BAK's therapeutic efficacy using micro\u2010CT and histopathological staining. We performed transcriptomic analysis and identified significantly enriched GO pathways. We then used biochemical assays and Western blotting to examine the effects of BAK on osteogenic differentiation\u2010related signaling factors in GIOP mice. We conducted in\u00a0vitro experiments using osteoblasts. We employed RT\u2010qPCR, Western blot, ALP &amp; ARS staining, immunofluorescence and dual\u2010luciferase reporter assays to assess BAK's influence on signaling pathways related to osteoblast differentiation. We used molecular docking, cellular thermal shift assay (CETSA), and drug affinity responsive target stability (DARTS) to confirm the direct binding interaction between BAK and FAT4. Finally, we silenced\n                    <jats:italic>Fat4<\/jats:italic>\n                    to validate that BAK exerts its anti\u2010GIOP effects by activating YAP1 through FAT4. BAK treatment significantly enhanced bone quality and strength in GIOP mice, and mitigated femoral pathological damage. BAK upregulated key osteoblast differentiation\u2010related transcription factors and enhanced serum alkaline phosphatase activity. Western blot confirmed that BAK increased protein levels of YAP1 while reducing levels of FAT4 and DCHS1. In\u00a0vitro, BAK similarly promoted osteoblast differentiation and activated YAP1 expression and\n                    <jats:italic>Runx2<\/jats:italic>\n                    transcription. Molecular docking, CETSA and DARTS analyses demonstrated that BAK directly binds to FAT4. Silencing\n                    <jats:italic>Fat4<\/jats:italic>\n                    abolished the pro\u2010osteogenic effects of BAK. BAK likely exerts its therapeutic effects in GIOP by targeting FAT4, activating YAP1 and promoting osteoblast differentiation.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70384","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T08:10:52Z","timestamp":1779264652000},"page":"5104-5118","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bakuchiol Ameliorates Glucocorticoid\u2010Induced Osteoporosis by Enhancing Osteoblast Differentiation via Targeting\n                    <scp>FAT4<\/scp>\n                    to Activate\n                    <scp>YAP1<\/scp>"],"prefix":"10.1002","volume":"40","author":[{"given":"Hengjun","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Orthopedics Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine in Osteoarthrosis Research Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Province Integrated Traditional Chinese and Western Medicine 3D Printing Technology Innovation Center Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine in Osteoarthrosis Research Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Province Integrated Traditional Chinese and Western Medicine 3D Printing Technology Innovation Center Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei 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Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Province Integrated Traditional Chinese and Western Medicine 3D Printing Technology Innovation Center Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Orthopedics Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine in Osteoarthrosis Research Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Province Integrated Traditional Chinese and Western Medicine 3D Printing Technology Innovation Center Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei 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TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Hebei Province Integrated Traditional Chinese and Western Medicine 3D Printing Technology Innovation Center Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1406-0292","authenticated-orcid":false,"given":"Zhongyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hebei Key Laboratory of Integrated Traditional Chinese and Western Medicine in Osteoarthrosis Research Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"},{"name":"Department of Diabetes Cangzhou Hospital of Integrated TCM\u2010WM  Cangzhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0820-5436","authenticated-orcid":false,"given":"Huantian","family":"Cui","sequence":"additional","affiliation":[{"name":"First School of Clinical Medicine, Yunnan 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