{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:07:32Z","timestamp":1791947252622,"version":"4.3.4"},"reference-count":51,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The necessity of iodinated contrast agents in radiographic diagnosis comes with an increased risk of kidney injury. The sclerotium of\n                    <jats:italic>Poria cocos<\/jats:italic>\n                    (Schw.) Wolf, a traditional Chinese medicine, exhibits anti\u2010inflammatory and diuretic pharmacological properties. Its extracted polysaccharides demonstrate anti\u2010apoptotic, antioxidant, and anti\u2010inflammatory activities. This study aimed to investigate the effects and potential mechanisms of\n                    <jats:italic>Poria cocos<\/jats:italic>\n                    polysaccharides (PCP) on contrast\u2010induced acute kidney injury (CI\u2010AKI). The purified PCP was characterized using FTIR and monosaccharide analysis. A CI\u2010AKI mouse model was established to evaluate its preventive effects via oral gavage. Mitochondrial structure, function, and biogenesis were assessed by measuring mitochondrial membrane potential (MMP), ROS, ATP, and mitochondrial DNA copy number. Antioxidant capacity and apoptosis levels were evaluated by detecting 8\u2010OHdG, caspase 3\/7 activity, cleaved\u2010PARP, MDA, and GSH. Finally, the mechanism of action of PCP was analyzed using qPCR and immunoblotting. The results showed that PCP significantly mitigated CI\u2010AKI by inhibiting contrast\u2010induced apoptosis and oxidative stress in kidney tissue. Furthermore, PCP maintained mitochondrial homeostasis in the kidneys by enhancing mitochondrial biogenesis and antioxidant capacity. These findings were consistent with in\u00a0vitro data. Notably, PGC\u20101\u03b1 was identified as a key factor in the nephroprotective effects of PCP, with its activation attributed to the CaMKK2\u2010AMPK signaling pathway. These findings suggest that PCP offers significant benefits in mitigating CI\u2010AKI by enhancing antioxidant defenses and promoting mitochondrial biogenesis through activation of the CaMKK2\/AMPK\/PGC\u20101\u03b1 signaling pathway in the kidney. Therefore, PCP could be a potential preventive agent for CI\u2010AKI.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70409","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T13:08:43Z","timestamp":1781788123000},"page":"5501-5518","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Poria cocos<\/i>\n                    Polysaccharides Protect Against Contrast\u2010Induced Renal Injury Through Activating\n                    <scp>CaMKK2<\/scp>\n                    \/\n                    <scp>AMPK<\/scp>\n                    \/\n                    <scp>PGC<\/scp>\n                    \u20101\u03b1 Signaling Pathway"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8668-7424","authenticated-orcid":false,"given":"Daolun","family":"Yu","sequence":"first","affiliation":[{"name":"College of Biotechnology and Pharmaceutical Engineering West Anhui 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