{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:18:11Z","timestamp":1788232691059,"version":"build-2803163510"},"reference-count":63,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204043"],"award-info":[{"award-number":["82204043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82473632"],"award-info":[{"award-number":["82473632"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2024JJ5458"],"award-info":[{"award-number":["2024JJ5458"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2025JJ50654"],"award-info":[{"award-number":["2025JJ50654"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Metabolic\u2010associated fatty liver disease (MAFLD), formerly known as non\u2010alcoholic fatty liver disease (NAFLD) has caused a significant health burden.\n                    <jats:italic>Glycyrrhiza uralensis Fisch<\/jats:italic>\n                    . is a traditional Chinese medicinal herb used to prevent and treat numerous diseases. Liquiritigenin (LQ), a flavonoid isolated from\n                    <jats:italic>Glycyrrhiza uralensis Fisch<\/jats:italic>\n                    ., has shown potential benefits in regulating lipid metabolism. This study aimed to analyze whether LQ was involved in ameliorating hepatic steatosis and elucidate the specific molecular mechanisms. A high\u2010fat diet (HFD) model of male C57BL\/6J mice was established to evaluate the effects of LQ on regulating hepatic steatosis. The potential molecular mechanisms of LQ on promoting lipid droplet catabolism were assessed in\u00a0vitro using HepG2 cells treated with palmitic acid (PA) and\/or LQ. The impact of the PLIN2\u2010ATGL signaling in lipid droplet catabolism was elucidated by treating HepG2 cells with NG\u2010497 (an ATGL inhibitor), the inhibitor of ATGL, and by constructing\n                    <jats:italic>Plin2<\/jats:italic>\n                    \u2010overexpressed HepG2 cell lines. Both in\u00a0vivo and in\u00a0vitro experiments demonstrated that LQ alleviated excessive lipid accumulation, enhanced lipolysis and lipophagy, down\u2010regulated PLIN2 expression and up\u2010regulated CGI\u201058 expression. Further, inhibiting lipolysis with NG\u2010497, the inhibitor of ATGL, attenuated the effects of LQ on increasing the phosphorylation of HSL and promoting lipophagy. The results also showed that transfecting HepG2 cells with a\n                    <jats:italic>Plin2<\/jats:italic>\n                    \u2010overexpressing vector reduced the effects of LQ on up\u2010regulating the expression of CGI\u201058, as well as proteins related to lipolysis and lipophagy. LQ promoted lipid droplet catabolism through the activation of lipolysis and lipophagy by regulating PLIN2\u2010ATGL signaling, which provided evidence for developing LQ as a functional compound in food for ameliorating hepatic steatosis.\n                  <\/jats:p>","DOI":"10.1002\/ptr.70440","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:12:02Z","timestamp":1788232322000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liquiritigenin Ameliorates High\u2010Fat Diet\u2010Induced Hepatic Steatosis by Promoting Lipid Droplet Catabolism via\n                    <scp>PLIN2\u2010ATGL<\/scp>\n                    Regulation"],"prefix":"10.1002","author":[{"given":"Zhuoya","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingmiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4578-5118","authenticated-orcid":false,"given":"Hong","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yansong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunqi","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhipeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4101-9408","authenticated-orcid":false,"given":"Wenya","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Food Hygiene, Xiangya School of Public Health Central South University  Changsha Hunan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2023.100461"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcp158"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10030352"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2021.1895658"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232415791"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2020.101635"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.32"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.1c10836"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvad095"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells10051016"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873\u20103468.14792"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374\u2010019\u20100327\u20105"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12944\u2010017\u20100473\u2010y"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.10.035"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2006.03.005"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v24.i30.3361"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2021.12326"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2024.110817"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051336"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M700359\u2010JLR200"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018\u2010022\u201004547\u20100"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11010091"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201500445"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.002675"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.4600"},{"issue":"2","key":"e_1_2_12_27_1","first-page":"301","article-title":"The Transcription Factor EB Links Cellular Stress to the Immune Response","volume":"90","author":"Nabar N. R.","year":"2017","journal-title":"Yale Journal of Biology and Medicine"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12060824"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.c22\u201000684"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580\u2010018\u20100085\u2010z"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24006"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0140\u20106736(20)32511\u20103"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2021.100973"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14061287"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.2741\/4892"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2023.2254191"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201803153"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12111015"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100039\u20101685524"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature07976"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202102_24875"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2017.07.009"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3699"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2017.1319544"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00098.2017"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010biochem\u2010061009\u2010102430"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2021.105937"},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2019.1659617"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23645"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401\u2010021\u201000786\u20102"},{"key":"e_1_2_12_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom12050672"},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1042\/bcj20190468"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu13020501"},{"key":"e_1_2_12_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2010.04.005"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00495"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14224910"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2017.109"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.30251"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29724"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154746"},{"key":"e_1_2_12_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u2010981\u201015\u20106082\u20108_13"},{"key":"e_1_2_12_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41574\u2010023\u201000845\u20100"},{"key":"e_1_2_12_63_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.851267"},{"key":"e_1_2_12_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112167"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70440","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.70440","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.70440","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T03:12:07Z","timestamp":1788232327000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.70440"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,31]]},"references-count":63,"alternative-id":["10.1002\/ptr.70440"],"URL":"https:\/\/doi.org\/10.1002\/ptr.70440","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,31]]},"assertion":[{"value":"2025-06-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"ptr.70440"}}