{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:44Z","timestamp":1786978424153,"version":"build-2736575974"},"reference-count":67,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,3,9]],"date-time":"2021-03-09T00:00:00Z","timestamp":1615248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972503"],"award-info":[{"award-number":["81972503"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:p>\n                    It is widely accepted that anaplastic thyroid carcinoma (ATC), a rare, extremely aggressive malignant, is enriched by cancer stem cells (CSCs), which are closely related to the pathogenesis of ATC. In the present study, we demonstrated that diallyl trisulphide (DATS), a well\u2010known hydrogen sulphide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) donor, suppressed sphere formation and restored the expression of iodide\u2010metabolizing genes in human ATC cells, which were associated with H\n                    <jats:sub>2<\/jats:sub>\n                    S generation. Two other H\n                    <jats:sub>2<\/jats:sub>\n                    S donors, NaHS and GYY4137, could also suppress the self\u2010renewal properties of ATC cells in vitro. Compared with normal thyroid tissues and papillary thyroid carcinomas (PTCs), the elevated expressions of SOX2 and MYC, two cancer stem cell markers, in ATCs were validated in the combined Gene Expression Omnibus (GEO) cohort. DATS decreased the expression of SOX2, which was mediated by H\n                    <jats:sub>2<\/jats:sub>\n                    S generation. Furthermore, knockdown of AKT or inhibition of AKT by DATS led to a decrease of SOX2 expression in ATC cells. AKT knockdown phenocopied restoration of thyroid\u2010specific gene expression in ATC cells. Our data suggest that H\n                    <jats:sub>2<\/jats:sub>\n                    S donors treatment can compromise the stem cell phenotype and restore thyroid\u2010specific gene expression of ATC cells by targeting AKT\u2010SOX2 pathway, which may serve as a therapeutic strategy to intervene the CSC progression of ATC.\n                  <\/jats:p>","DOI":"10.1002\/ptr.7065","type":"journal-article","created":{"date-parts":[[2021,3,10]],"date-time":"2021-03-10T01:06:03Z","timestamp":1615338363000},"page":"3428-3443","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Diallyl trisulphide, a\n                    <scp>\n                      H\n                      <sub>2<\/sub>\n                      S\n                    <\/scp>\n                    donor, compromises the stem cell phenotype and restores thyroid\u2010specific gene expression in anaplastic thyroid carcinoma cells by targeting\n                    <scp>AKT\u2010SOX2<\/scp>\n                    axis"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1740-8833","authenticated-orcid":false,"given":"Li","family":"Zhang","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi China"},{"name":"Department of Radiopharmaceuticals, School of Pharmacy Nanjing Medical University  Nanjing China"},{"name":"School of Life Science and Technology Southeast University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shichen","family":"Xu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Nuclear Medicine, Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine  Wuxi 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