{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T01:51:37Z","timestamp":1781229097351,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2021,8,22]],"date-time":"2021-08-22T00:00:00Z","timestamp":1629590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1127156"],"award-info":[{"award-number":["1127156"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1027932"],"award-info":[{"award-number":["1027932"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1107828"],"award-info":[{"award-number":["1107828"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ21H060010"],"award-info":[{"award-number":["LQ21H060010"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010248","name":"Zhejiang Province Public Welfare Technology Application Research Project","doi-asserted-by":"publisher","award":["LGF20H060013"],"award-info":[{"award-number":["LGF20H060013"]}],"id":[{"id":"10.13039\/501100010248","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871806"],"award-info":[{"award-number":["81871806"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2021,10]]},"abstract":"<jats:p>Morin is a natural compound isolated from moraceae family members and has been reported to possess a range of pharmacological activities. However, the effects of morin on bone\u2010associated disorders and the potential mechanism remain unknown. In this study, we investigated the anti\u2010osteoclastogenic effect of morin in vitro and the potential therapeutic effects on ovariectomy (OVX)\u2010induced osteoporosis in vivo. In vitro, by using a bone marrow macrophage\u2010derived osteoclast culture system, we determined that morin attenuated receptor activator of nuclear factor (NF)\u2010\u03baB ligand (RANKL)\u2010induced osteoclast formation via the inhibition of the mitogen\u2010activated protein kinase (MAPK), NF\u2010\u03baB and calcium pathways. In addition, the subsequent expression of nuclear factor of activated T cells c1 (NFATc1) and c\u2010fos was significantly suppressed by morin. In addition, NFATc1 downregulation led to the reduced expression of osteoclastogenesis\u2010related marker genes, such as V\u2010ATPase\u2010d2 and Integrin \u03b23. In vivo, results provided that morin could effectively attenuate OVX\u2010induced bone loss in C57BL\/6 mice. In conclusion, our results demonstrated that morin suppressed RANKL\u2010induced osteoclastogenesis via the NF\u2010\u03baB, MAPK and calcium pathways, in addition, its function of preventing OVX\u2010induced bone loss in vivo, which suggested that morin may be a potential therapeutic agent for postmenopausal osteoporosis treatment.<\/jats:p>","DOI":"10.1002\/ptr.7229","type":"journal-article","created":{"date-parts":[[2021,8,23]],"date-time":"2021-08-23T06:50:10Z","timestamp":1629701410000},"page":"5694-5707","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Morin attenuates osteoclast formation and function by suppressing the <scp>NF\u2010\u03baB<\/scp>, <scp>MAPK<\/scp> and calcium signalling pathways"],"prefix":"10.1002","volume":"35","author":[{"given":"Yifeng","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"},{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"},{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiwei","family":"Shen","sequence":"additional","affiliation":[{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianchen","family":"Qian","sequence":"additional","affiliation":[{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youjin","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Endocrinology The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Jiang","sequence":"additional","affiliation":[{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghao","family":"Lin","sequence":"additional","affiliation":[{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Liu","sequence":"additional","affiliation":[{"name":"The Second School of Medicine Wenzhou Medical University  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaosen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiake","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"},{"name":"School of Biomedical Sciences The University of Western Australia  Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2666-8415","authenticated-orcid":false,"given":"Haiming","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University  Wenzhou China"},{"name":"Key Laboratory of Orthopaedics of Zhejiang Province  Wenzhou China"},{"name":"School of Biomedical Sciences The University of Western Australia  Perth Western Australia Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,8,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2006.09.023"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2018.07.035"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1914-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.06.059"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-1136-2"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2016.11.009"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.201801671"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2018.3603"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2018.8935"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu10070901"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14713"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","unstructured":"Jules J. Chen W. Feng X. &Li Y.(2018).C\/EBP\u03b1 transcription factor is regulated by the RANK cytoplasmic535IVVY538motif and stimulates osteoclastogenesis more strongly than c\u2010Fos 293(4) 1480\u20131492.https:\/\/doi.org\/10.1074\/jbc.M116.736009","DOI":"10.1074\/jbc.M116.736009"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b05147"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b00233"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.14348\/molcells.2019.0232"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm7100345"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8040431"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0800642105"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.06.161"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23764"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2017.2871"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.07217"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14490"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061498"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs407"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep36662"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.35414"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/5843672"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2295"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00821.x"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2016.41"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000362647"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.02.035"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.2337\/db09-0232"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2017.03.009"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00360"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.127571"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9050441"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-018-1228-4"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13942"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.3077"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2000.15.11.2178"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2807"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2020.00348"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.10785"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2002979"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7229","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.7229","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7229","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T23:52:06Z","timestamp":1692834726000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.7229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,22]]},"references-count":46,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["10.1002\/ptr.7229"],"URL":"https:\/\/doi.org\/10.1002\/ptr.7229","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,22]]},"assertion":[{"value":"2020-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-07-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}