{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:00:50Z","timestamp":1774494050533,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874324"],"award-info":[{"award-number":["81874324"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Nonalcoholic fatty liver disease (NAFLD), a metabolic disease, has received wide attention worldwide. However, there is no approved effective drug for NAFLD treatment. In the study, H&amp;E and Oil Red O staining were employed to detect liver histopathological changes and the accumulation of lipid droplets. Quantitative real\u2010time PCR, Western blot, bioinformatics, luciferase assay, immunofluorescence staining, reactive oxygen species (ROS), and siRNA were used to further elucidate the mechanism of isoliquiritigenin (ISL) against NAFLD. The results showed that ISL significantly reduced the liver\u2010to\u2010body weight ratios and biochemical index. And the staining results showed that ISL remarkedly ameliorated liver histopathological changes of NAFLD. Furthermore, ISL significantly increased the levels of PPAR\u03b1, CPT1\u03b1, and ACADS, which were involved in lipid metabolism, and inhibited the ROS, TNF\u2010\u03b1, IL\u20101\u03b2, and IL\u20106 expression by activating PGC\u20101\u03b1. Bioinformatics and luciferase assay analysis confirmed that miR\u2010138\u20105p might bind to PGC\u20101\u03b1 mRNA in NAFLD. Importantly, the expression of miR\u2010138\u20105p was increased in the NAFLD, which was significantly decreased by ISL. In addition, the miR\u2010138\u20105p inhibitor also promoted lipid metabolism and inhibited inflammatory response in NAFLD via PGC\u20101\u03b1 activation. The above results demonstrate that ISL alleviates NAFLD through modulating miR\u2010138\u20105p\/PGC\u20101\u03b1\u2010mediated lipid metabolism and inflammatory reaction in vivo and in vitro.<\/jats:p>","DOI":"10.1002\/ptr.7334","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T13:55:45Z","timestamp":1642514145000},"page":"899-913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Activation of <scp>PGC<\/scp>\u20101\u03b1 via isoliquiritigenin\u2010induced downregulation of <scp>miR<\/scp>\u2010138\u20105p alleviates nonalcoholic fatty liver disease"],"prefix":"10.1002","volume":"36","author":[{"given":"Lu","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacology College of Pharmacy, Dalian Medical University  Dalian 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