{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T06:07:26Z","timestamp":1768802846852,"version":"3.49.0"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102019R1I1A2A01063598"],"award-info":[{"award-number":["NRF\u20102019R1I1A2A01063598"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Phlomis umbrosa<\/jats:italic> has been traditionally used for bone diseases in traditional Korean Medicine. Sweroside (SOS), marker compounds of <jats:italic>P. umbrosa<\/jats:italic>, has been known to promote osteoblast differentiation. In this study, ameliorative effects of SOS on osteoporosis and potential target pathway were investigated. Ovariectomized mice were administered three doses of SOS three times a week for 4\u2009weeks after inducing osteoporosis. Bone mineral content (BMC) and bone mineral density (BMD) were analyzed by dual energy X\u2010ray absorptiometry. A human osteosarcoma cell line (SaOS\u20102) was differentiated to clarify the promoting effects of SOS on osteoblast differentiation and bone formation. Osteoblastic bone\u2010forming markers were evaluated in lumbar vertebrae (LV) and mineralized SaOS\u20102 cells. SOS markedly elevated BMC and BMD levels and attenuated the bone marrow adipocytes in the femoral shaft. SOS increased the formation of bone matrix in SaOS\u20102 cells. Bone morphogenetic protein\u20102 (<jats:italic>BMP2<\/jats:italic>) and runt\u2010related transcription factor 2 (<jats:italic>CBFA1<\/jats:italic>) in LV and SaOS\u20102 cells were up\u2010regulated by SOS. SOS increased alkaline phosphatase (<jats:italic>ALPL<\/jats:italic>), osteopontin (<jats:italic>SPP1<\/jats:italic>), and bone sialoprotein\u20101 (<jats:italic>BSPH1<\/jats:italic>). In conclusion, SOS induced the formation of mineralized bone matrix by regulating <jats:italic>BMP2\/CBFA1<\/jats:italic>\u2010mediated molecules. Therefore, SOS could be a therapeutic compound of treatment for osteoporosis by producing the new bone matrix.<\/jats:p>","DOI":"10.1002\/ptr.7336","type":"journal-article","created":{"date-parts":[[2021,11,26]],"date-time":"2021-11-26T08:32:23Z","timestamp":1637915543000},"page":"7050-7063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Promotion of osteogenesis by Sweroside via <scp><i>BMP2<\/i><\/scp>\u2010involved signaling in postmenopausal osteoporosis"],"prefix":"10.1002","volume":"35","author":[{"given":"La Yoon","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Convergence Korean Medical Science, College of Korean Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi Hye","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Convergence Korean Medical Science, College of Korean Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5308-2386","authenticated-orcid":false,"given":"Woong Mo","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Convergence Korean Medical Science, College of Korean Medicine Kyung Hee University  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nu.10.070190.002145"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.11138\/ccmbm\/2015.12.2.111"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2017.00234"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.TEB.2016.0454"},{"issue":"4","key":"e_1_2_8_6_1","first-page":"324","article-title":"Bisphosphonates for treatment of osteoporosis: Expected benefits, potential harms, and drug holidays","volume":"60","author":"Brown J. 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