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The aim of this study was to evaluate the antiinflammatory and analgesic potentials of <jats:italic>Nigella orientalis<\/jats:italic> L. seeds fixed oil (NOO). The acetic acid writhing test and the formaldehyde\u2010induced licking paw were performed to assess the analgesic activity of the oil. The antiinflammatory activity was first evaluated in vitro by the erythrocyte membrane stabilization then in vivo by xylene\u2010 and carrageenan\u2010induced ear and paw edema, respectively. To further understand the molecular mechanism of action of the Nigella extract, lipopolysaccharide\u2010activated RAW 264.7 macrophages were used. Nitric oxide (NO) production was measured by Griess reaction and cell viability by MTT assay. The gene and protein expression of inflammatory mediators were assessed by RT\u2010PCR and western blot, respectively. NOO exerted a potent analgesic effect in in vivo models of writhing test and induced edema. The analyzed molecular mechanisms revealed a role for NO and prostaglandins as molecules mediating the pharmacological effects of the extract through a mechanism involving nuclear factor\u2010\u03baB and mitogen\u2010activated protein kinases. This study demonstrates, for the first time, that the fixed oil of <jats:italic>N. orientalis<\/jats:italic> has strong antinociceptive and antiinflammatory properties and might be a promising agent for the treatment of certain inflammation\u2010related diseases.<\/jats:p>","DOI":"10.1002\/ptr.7400","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T12:53:29Z","timestamp":1645620809000},"page":"1372-1385","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Analgesic and antiinflammatory effects of <i>Nigella orientalis<\/i> L. seeds fixed oil: Pharmacological potentials and molecular mechanisms"],"prefix":"10.1002","volume":"36","author":[{"given":"Djedjiga","family":"Ait Eldjoudi","sequence":"first","affiliation":[{"name":"Laboratoire de Biotechnologies V\u00e9g\u00e9tales et Ethnobotanique, Facult\u00e9 des Sciences de la Nature et de la Vie Universit\u00e9 de Bejaia  Bejaia 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