{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T19:30:52Z","timestamp":1792438252924,"version":"4.5.0"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Cardamonin is a chalcone with neuroprotective activity. The aim of our study was to explore the functions and mechanism of action of cardamonin in ischemic stroke. Oxygen\u2013glucose deprivation and reperfusion (OGD\/R)\u2010induced human brain microvascular endothelial cells (HBMECs) and middle cerebral artery occlusion (MCAO) mouse model were utilized to mimic ischemic stroke. Cell viability was analyzed by 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl\u20102H\u2010tetrazolium bromide. Permeability was investigated via fluorescein isothiocyanate\u2010dextran assay. Apoptosis was detected by TdT\u2010Mediated dUTP Nick End Labeling staining. Hypoxia\u2010inducible factor (HIF)\u20101\u03b1 and vascular endothelial growth factor A (VEGFA) protein levels were measured using Western blotting. Brain injury was evaluated by 2,3,5\u2010triphenyltetrazolium chloride staining, neurological score and brain water content. The 37 overlapping targets of ischemic stroke and cardamonin were predicted to be associated with the HIF\u20101\/VEGFA signaling. Cardamonin alleviated OGD\/R\u2010induced viability reduction and increase of permeability and apoptosis in HBMECs. Cardamonin increased OGD\/R\u2010induced activation of the HIF\u20101\u03b1\/VEGFA pathway. Inhibition of the HIF\u20101\u03b1\/VEGFA signaling using inhibitor relieved the effect of cardamonin on cell viability, permeability and apoptosis in HBMECs under OGD\/R. Cardamonin mitigated brain injury and promoted activation of the HIF\u20101\u03b1\/VEGFA signaling in MCAO\u2010treated mice. Overall, cardamonin protected against OGD\/R\u2010induced HBMEC damage and MACO\u2010induced brain injury through activating the HIF\u20101\u03b1\/VEGFA pathway.<\/jats:p>","DOI":"10.1002\/ptr.7409","type":"journal-article","created":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T07:34:31Z","timestamp":1644478471000},"page":"1736-1747","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Cardamonin attenuates cerebral ischemia\/reperfusion injury by activating the\n                    <scp>HIF<\/scp>\n                    \u20101\u03b1\/\n                    <scp>VEGFA<\/scp>\n                    pathway"],"prefix":"10.1002","volume":"36","author":[{"given":"Hongzao","family":"Ni","sequence":"first","affiliation":[{"name":"Department of Neurosurgery The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an  Huai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Xinyi People's Hospital  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyu","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Affiliated Huai'an Hospital of Xuzhou Medical University, The Second People's Hospital of Huai'an  Huai'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9175-6011","authenticated-orcid":false,"given":"Botao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The Second Affiliated Hospital of Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-020-01237-y"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00095.2018"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0449-07.2007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-15-293"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2018.09.012"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15129104"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.918626"},{"issue":"48","key":"e_1_2_7_9_1","first-page":"2761","article-title":"Mouse model of middle cerebral artery occlusion","volume":"13","author":"Chiang T.","year":"2011","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz382"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2013.04.024"},{"key":"e_1_2_7_12_1","first-page":"3445","article-title":"Animal models of ischemic stroke and their application in clinical research","volume":"9","author":"Fluri F.","year":"2015","journal-title":"Drug Design, Development and Therapy"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.28920\/dhm47.2.110-117"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009767"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2021.119525"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkn923"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-020-00295-2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pneurobio.2017.10.001"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-019-1351-4"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(00)00207-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2013.07.060"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1649503"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S77115"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/1567202614666171128120558"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2019.04.031"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-004-1384-9"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7FO00054E"},{"issue":"1","key":"e_1_2_7_29_1","first-page":"D845","article-title":"The DisGeNET knowledge platform for disease genomics: 2019 update","volume":"48","author":"Pinero J.","year":"2020","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109547"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-014-9531-7"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.104184"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnins.2021.555543"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2020.11.008"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2014.11.002"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1131"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-0397-3"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6660193"},{"issue":"4","key":"e_1_2_7_39_1","first-page":"2685","article-title":"Long noncoding RNA SNHG16 inhibits the oxygenglucose deprivation and reoxygenationinduced apoptosis in human brain microvascular endothelial cells by regulating miR\u201015a\u20105p\/bcl2","volume":"22","author":"Teng H.","year":"2020","journal-title":"Molecular Medicine Reports"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389203719666180104113937"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2018.07.039"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2017.12.010"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.50674"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30500-3"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02187-y"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.4103\/1673-5374.170324"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1208\/s12248-015-9854-1"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.04.022"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12883-019-1508-y"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7409","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.7409","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T06:30:49Z","timestamp":1692945049000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.7409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,10]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/ptr.7409"],"URL":"https:\/\/doi.org\/10.1002\/ptr.7409","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,10]]},"assertion":[{"value":"2021-06-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}