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Medicinal plants are renowned as a complementary approach in which antiviral natural products have been established as safe and effective drugs. Here, we report that the percolation extract of <jats:italic>Spatholobus suberectus<\/jats:italic> Dunn (SSP) is a broad\u2010spectrum viral entry inhibitor against SARS\u2010CoV\u20101\/2 and other enveloped viruses. The viral inhibitory activities of the SSP were evaluated by using pseudotyped SARS\u2010CoV\u20101 and 2, HIV\u20101<jats:sub>ADA and HXB2<\/jats:sub>, and H5N1. SSP effectively inhibited viral entry and with EC<jats:sub>50<\/jats:sub> values ranging from 3.6 to 5.1\u2009\u03bcg\/ml. Pre\u2010treatment of pseudovirus or target cells with SSP showed consistent inhibitory activities with the respective EC<jats:sub>50<\/jats:sub> value of 2.3 or 2.1\u2009\u03bcg\/ml. SSP blocked both SARS\u2010CoV\u20102 spike glycoprotein and the host ACE2 receptor. <jats:italic>In vivo<\/jats:italic> studies indicated that there was no abnormal toxicity and behavior in long\u2010term SSP treatment. Based on these findings, we concluded that SSP has the potential to be developed as a drug candidate for preventing and treating COVID\u201019 and other emerging enveloped viruses.<\/jats:p>","DOI":"10.1002\/ptr.7452","type":"journal-article","created":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T12:43:57Z","timestamp":1660049037000},"page":"3232-3247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Broad\u2010spectrum antiviral activity of <i>Spatholobus suberectus<\/i> Dunn against <scp>SARS\u2010CoV<\/scp>\u20102, <scp>SARS\u2010CoV<\/scp>\u20101, <scp>H5N1<\/scp>, and other enveloped viruses"],"prefix":"10.1002","volume":"36","author":[{"given":"Qingqing","family":"Liu","sequence":"first","affiliation":[{"name":"School of Chinese Medicine, Li Ka Shing Faculty of Medicine The University of Hong Kong  Hong Kong China"},{"name":"Shenzhen Institute of Research and Innovation University of Hong Kong  Shenzhen 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