{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T12:08:25Z","timestamp":1782821305920,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T00:00:00Z","timestamp":1651017600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    Mill. is an edible plant belonging to the\n                    <jats:italic>Brassicaceae<\/jats:italic>\n                    botanical family with a long story as a medicinal material, mainly linked to the presence of glucoerucin. One of the main products of this glucosinolate is erucin, a biologicallly active isothiocyanate recently recognized as a hydrogen sulfide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) donor. In this work, an\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    extract has been obtained from a defatted seed meal (DSM), achieving a powder rich in thiofunctionalized glucosinolates, glucoerucin, and glucoraphanin, accounting for 95% and 5% of the total glucosinolate content (17% on a dry weight basis), associated with 13 identified phenolic acids and flavonoids accounting for 2.5%. In a cell\u2010free model,\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    DSM extract slowly released H\n                    <jats:sub>2<\/jats:sub>\n                    S. Moreover, this extract promoted significant hypotensive effects in hypertensive rats, and evoked dose\u2010dependent cardioprotection in in vivo model of acute myocardial infarct, obtained through a reversible coronary occlusion. This latter effect was sensitive to blockers of mitochondrial KATP and Kv7.4 potassium channels, suggesting a potential role of these mitochondrial channels in the protective effects of\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    DSM extract. Accordingly,\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    DSM extract reduced calcium uptake and apoptotic cell death in isolated cardiac mitochondria. Taken together, these results demonstrate that\n                    <jats:italic>Eruca sativa<\/jats:italic>\n                    DSM extract is endowed with an interesting nutraceutical profile on the cardiovascular system due to, at least in part, its H\n                    <jats:sub>2<\/jats:sub>\n                    S releasing properties. These results pave the way for future investigations on active metabolites.\n                  <\/jats:p>","DOI":"10.1002\/ptr.7479","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:52:28Z","timestamp":1651107148000},"page":"2616-2627","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Cardiovascular benefits of\n                    <i>Eruca sativa<\/i>\n                    mill. Defatted seed meal extract: Potential role of hydrogen sulfide"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2431-6248","authenticated-orcid":false,"given":"Lara","family":"Testai","sequence":"first","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Center Nutrafood \u201cNutraceuticals and Food for Health\u201d University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Centre of Ageing Biology and Pathology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Pagnotta","sequence":"additional","affiliation":[{"name":"CREA\u2010Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenia","family":"Piragine","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Flori","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Citi","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2090-7107","authenticated-orcid":false,"given":"Alma","family":"Martelli","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Center Nutrafood \u201cNutraceuticals and Food for Health\u201d University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Centre of Ageing Biology and Pathology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8374-4432","authenticated-orcid":false,"given":"Lorenzo","family":"Di Cesare Mannelli","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology, Drug Research and Child Health\u2013Neurofarba\u2013 Pharmacology and Toxicology Section University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Ghelardini","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Psychology, Drug Research and Child Health\u2013Neurofarba\u2013 Pharmacology and Toxicology Section University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Matteo","sequence":"additional","affiliation":[{"name":"CREA\u2010Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serafino","family":"Suriano","sequence":"additional","affiliation":[{"name":"CREA\u2010Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Troccoli","sequence":"additional","affiliation":[{"name":"CREA\u2010Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Pecchioni","sequence":"additional","affiliation":[{"name":"CREA\u2010Council for Agricultural Research and Economics, Research Centre for Cereal and Industrial Crops  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1441-5421","authenticated-orcid":false,"given":"Vincenzo","family":"Calderone","sequence":"additional","affiliation":[{"name":"Department of Pharmacy University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Center Nutrafood \u201cNutraceuticals and Food for Health\u201d University of Pisa  Pisa Italy"},{"name":"Interdepartmental Research Centre of Ageing Biology and Pathology University of Pisa  Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7314\/apjcp.2015.16.18.8411"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-021-14532-y"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf047945a"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fochx.2018.100002"},{"key":"e_1_2_10_6_1","volume-title":"Rocket: A Mediterranean crop for the world","author":"Bhandari D. C.","year":"1996"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450116666151001112020"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0034-1368591"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867325666180212120504"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.05.031"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2018.01.022"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2015.09.001"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu6125839"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.8695"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2014.02.012"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182137"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/2156587214527452"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2002.06.001"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6159"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24163006"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14645"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10060961"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.20234"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2013.11.003"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfpe.13704"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22020961"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/918069"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6941"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00170.2006"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.06.013"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2006.11.013"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.12.022"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e318180ff89"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000539-199809000-00014"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.09.006"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21332"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.12.021"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-020-10083-8"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.02.017"},{"key":"e_1_2_10_40_1","first-page":"257","article-title":"Guidelines for glucosinolate analysis in green tissues used for biofumigation","volume":"3","author":"Wathelet J. P.","year":"2004","journal-title":"Agroindustria"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402004101"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2179021"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7479","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.7479","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7479","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T14:18:44Z","timestamp":1764512324000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.7479"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,27]]},"references-count":41,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1002\/ptr.7479"],"URL":"https:\/\/doi.org\/10.1002\/ptr.7479","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,27]]},"assertion":[{"value":"2021-12-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}