{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T04:50:47Z","timestamp":1789015847277,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T00:00:00Z","timestamp":1654473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The polysaccharide extract from <jats:italic>Isatidis Radix<\/jats:italic> exhibits potent antiinflammatory and antiviral activities, but the mechanism of <jats:italic>Isatidis Radix<\/jats:italic> polysaccharide (IRP) remains obscure. Herein, we reported that IRP blocked the activation of nod\u2010like receptor pyrin domain\u2010containing 3 (NLRP3) inflammasome, leading to the inhibiting of caspase\u20101 cleavage and IL\u20101\u03b2 secretion. Mechanistically, IRP did not inhibit NLRP3 inflammasome through suppressing mitochondrial reactive oxygen species <jats:bold>(<\/jats:bold>mtROS) production. However, IRP can significantly suppress the oligomerization of apoptosis\u2010associated speck\u2010like protein (ASC) and subsequently block the formation of inflammasome. Next, we evaluate the role of IRP in monosodium urate (MSU)\u2010induced gout in vivo which is a NLRP3\u2010associated disease. We also observed that oral administration of IRP can reduce the increased ankle thickness and the secretion of IL\u20101\u03b2, IL\u201018, IL\u20106, TNF\u2010\u03b1 and MPO of the mouse ankle joints caused by MSU crystals. Furthermore, flow cytometry analysis highlighted a significant modulation of T helper 17 cells (Th17)\/regulatory T cells (Treg) following IRP treatment in MSU induced gout. Overall, our findings suggest that IRP has comprehensive and potent antiinflammatory effects and provide a reasonable therapeutic strategy in preventing inflammasome\u2010associated diseases, such as inflammatory gouty arthritis.<\/jats:p>","DOI":"10.1002\/ptr.7514","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T18:04:01Z","timestamp":1654538641000},"page":"3295-3312","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Polysaccharide extract from <scp><i>Isatidis Radix<\/i><\/scp> inhibits multiple inflammasomes activation and alleviate gouty arthritis"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1032-8635","authenticated-orcid":false,"given":"Lutong","family":"Ren","sequence":"first","affiliation":[{"name":"School of Chinese Materia Medica Beijing University of Chinese Medicine  Beijing China"},{"name":"Department of Hepatology Fifth 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