{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T08:07:10Z","timestamp":1748246830818,"version":"3.40.5"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T00:00:00Z","timestamp":1654041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>2,3,5,4\u2019\u2010Tetrahydrostilbene\u20102\u2010o\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucoside (TSG) is the main active component of <jats:italic>Polygonum multiflorum Thunb<\/jats:italic>. It has effects on hypertension. However, the mechanism is unclear. Current research is devoted to exploring the mechanism of TSG improving HHcy\u2010induced hypertension. The mice received a subcutaneous injection of Hcy in the presence or absence of TSG for 4\u2009weeks. Blood pressure (BP) was measured using a noninvasive tail\u2010cuff plethysmography method. Levels of plasma Hcy and endothelin\u20101 were measured using ELISA. Rat SMA without endothelium was cultured in a serum\u2010free medium in the presence or absence of TSG with or without Hcy. The contractile response to sarafotoxin 6c or endothein\u20101 was studied using a sensitive myography. The levels of protein were detected using Western blotting. The results showed that TSG lowered HHcy\u2010elevated BP and decreased levels of plasma Hcy and endothelin\u20101 in mice. Furthermore, the results showed that TSG inhibited Hcy\u2010upregulated ET receptor expression and ET receptor\u2010mediated contractile responses as well as the levels of p\u2010ERK<jats:sub>1\/2<\/jats:sub> and p\u2010p65 in SMA. In vivo results further validate the in vitro results. In conclusion, TSG can decrease the levels of plasma Hcy and ET\u20101 and downregulate Hcy\u2010upregulated ET receptors in VSMCs by inhibiting the ERK<jats:sub>1\/2<\/jats:sub>\/NF\u2010\u03baB\/ET<jats:sub>B2<\/jats:sub> pathway to lower the BP.<\/jats:p>","DOI":"10.1002\/ptr.7519","type":"journal-article","created":{"date-parts":[[2022,6,1]],"date-time":"2022-06-01T15:35:52Z","timestamp":1654097752000},"page":"3352-3361","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Tetrahydroxystilbene glucoside attenuated homocysteine\u2010upregulated endothelin receptors in vascular smooth muscle cells via the <scp>ERK<sub>1<\/sub><\/scp><sub>\/2<\/sub>\/<scp>NF\u2010\u03baB<\/scp> signaling pathway"],"prefix":"10.1002","volume":"36","author":[{"given":"Min","family":"Jia","sequence":"first","affiliation":[{"name":"Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingli","family":"Su","sequence":"additional","affiliation":[{"name":"School of Basic and Medical Sciences Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiaohong","family":"Qin","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajuan","family":"Li","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1888-4716","authenticated-orcid":false,"given":"Siwang","family":"Wang","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Biomedicine, School of Life Sciences Northwest University  Xi'an Shaanxi China"},{"name":"Institute of Materia Medic, Department of Natural Medicine School of Pharmacy Air Force Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1896-7194","authenticated-orcid":false,"given":"Yulong","family":"Chen","sequence":"additional","affiliation":[{"name":"Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine Xi'an Medical University  Xi'an Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,6]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2016.08.011"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2016.3605"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.11.011"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2017.05.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2018.03.010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109758"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001177"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.115.011833"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-019-0176-3"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2013.12.209"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X16500786"},{"first-page":"176","volume-title":"Editorial Committee of Chinese Pharmacopoeia","year":"2015","key":"e_1_2_9_13_1"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00088.2016"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.107.094821"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i12.1699"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1124\/jpet.110.175380"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/2047487314522136"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mvr.2011.12.001"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2011.12.001"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17966"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e32805bf8ed"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.115.07031"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0701054"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2017.05.006"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0895-7061(01)02074-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mehy.2004.10.017"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.112.044545"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.325.7374.1202"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.02.027"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/1076029613519849"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291x(91)91601-8"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2013.11.004"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfn173"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2010.04.013"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X15500123"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1159\/000366349"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxlet.2011.12.005"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7519","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.7519","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7519","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T13:44:18Z","timestamp":1692711858000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.7519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["10.1002\/ptr.7519"],"URL":"https:\/\/doi.org\/10.1002\/ptr.7519","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"type":"print","value":"0951-418X"},{"type":"electronic","value":"1099-1573"}],"subject":[],"published":{"date-parts":[[2022,6]]},"assertion":[{"value":"2020-08-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}