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Immune dysfunction involving dendritic cells (DCs) and T cells is recognized as a key factor in diseases. Indirubin (IDRB) exerts antiinflammatory effects and can help in treating immune diseases. This study aimed to isolate bone marrow\u2010derived dendritic cells (BMDCs) using lipopolysaccharide\u00a0(LPS) to obtain mature DCs (mDCs). The expression of CD80, CD86, CD40, and MHC\u2010II was detected using flow cytometry after treatment with IDRB. \u03b1V\u03b28 siRNA was used to knock down \u03b1V\u03b28 in mDCs, and the expression of CD80, CD86, CD40, and MHC\u2010II was detected. Meanwhile, DCs were co\u2010cultured with T cells. Then, T cell differentiation was detected using flow cytometry, and the cytokine levels were detected using enzyme\u2010linked immunosorbent assay. The animal model of dextran sulfate sodium (DSS)\u2010induced inflammatory bowel disease was established in mice. After intervention with IDRB and \u03b1V\u03b28 shRNA, the intestinal tissues were evaluated using H&amp;E staining, disease activity index (DAI) score, and histological damage index, and the corresponding factors and cytokines to regulatory T cells (Treg) and Th17 were measured. The results showed that \u03b1V\u03b28 was expressed in immature DCs and mDCs. CD80, CD86, CD40, and MHC\u2010II expression decreased after IDRB treatment in mDCs. Meanwhile, the expression of TNF\u2010\u03b1 and TGF\u2010\u03b2 also decreased after IDRB treatment. The effect of IDRB on the expression of CD80, CD86, CD40, MHC\u2010II, TNF\u2010\u03b1, and TGF\u2010\u03b2 in mDCs was reversed by \u03b1V\u03b28 siRNA. The Treg differentiation increased after IDRB treatment, while the differentiation of Th17 cells was inhibited. This effect of IDRB was reversed by mDCs after treatment with \u03b1V\u03b28 siRNA. In vivo experiments showed that IDRB alleviated the symptoms of inflammatory bowel disease in animals. Enteritis significantly reduced, and the effect of IDRB was reversed by \u03b1V\u03b28 shRNA. The results suggested that IDRB regulated the differentiation of T cells by mediating the maturation of BMDCs through \u03b1V\u03b28. This study confirmed the therapeutic effect of IDRB in inflammatory bowel disease and suggested that IDRB might serve as a potential drug.<\/jats:p>","DOI":"10.1002\/ptr.7595","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:20:15Z","timestamp":1664151615000},"page":"89-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Indirubin regulates T cell differentiation by promoting \u03b1V\u03b28 expression in bone marrow\u2010derived dendritic cells to alleviate inflammatory bowel disease"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5907-5312","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Nanchong Central Hospital, The Second Clinical Medical College, North Sichuan Medical College  Nanchong Sichuan 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