{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T03:53:40Z","timestamp":1781495620767,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,9,25]],"date-time":"2022-09-25T00:00:00Z","timestamp":1664064000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The emergence of methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) has become a critical global concern. Identifying new anti\u2010<jats:italic>S. aureus<\/jats:italic> agents or therapeutic strategies are urgently needed to treat <jats:italic>S. aureus<\/jats:italic> infection. The present study investigated the antibacterial activity of 16 phenolic compounds against MRSA, four of which exhibited antibacterial activity. Their antibacterial activities increased in a dose\u2010dependent manner but showed different responses with the extension of treatment time. Trialdehyde phloroglucinol (TPG) and 2\u2010nitrophloroglucinol (NPG) maintained stable antibacterial activity; however, that of dichlorophenol and myricetin decreased rapidly over 24\u2009hr of treatment. Checkerboard and time\u2010kill assays indicated that TPG and NPG exhibited strong synergistic antibacterial activities with penicillin or bacitracin. Microscopic observation and membrane integrity analysis showed that the combination of TPG and penicillin destroyed the MRSA cell membrane, resulting in the leakage of intracellular biomacromolecules, marked changes in surface zeta potential, and the collapse of membrane potential. Moreover, the combination significantly decreased penicillinase activity and penicillin\u2010binding protein 2a mRNA expression, inhibiting MRSA growth. Taken together, these results demonstrated that the combination of the phloroglucinol derivative TPG and penicillin has significant synergistic anti\u2010MRSA activity and can serve as a potential therapeutic strategy to treat MRSA infections.<\/jats:p>","DOI":"10.1002\/ptr.7629","type":"journal-article","created":{"date-parts":[[2022,9,26]],"date-time":"2022-09-26T00:54:25Z","timestamp":1664153665000},"page":"490-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Antibacterial activity and synergistic antibiotic mechanism of trialdehyde phloroglucinol against methicillin\u2010resistant <i>Staphylococcus aureus<\/i>"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4744-3867","authenticated-orcid":false,"given":"Dingyi","family":"Shen","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine South China Agricultural University  Guangzhou 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