{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:10:37Z","timestamp":1781014237478,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T00:00:00Z","timestamp":1665532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81330081"],"award-info":[{"award-number":["81330081"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81503084"],"award-info":[{"award-number":["81503084"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peoniflorin\u20106\u2032\u2010O\u2010benzene sulfonate (CP\u201025) inhibited the activity of GRK2 to exert anti\u2010inflammatory and immunomodulatory effects. This study aimed to investigate the effect of CP\u201025 the intestinal epithelial barrier and the mechanism. CaCO\u20102 cell monolayer and dextran sulfate salt (DSS)\u2010induced colitis mouse model was used to evaluate intestinal epithelial barrier function in vitro and in vivo, respectively. Results showed that CP\u201025 prevented dysfunction of the intestinal epithelial barrier and inhibited NF\u2010\u03baB p65 activation in TNF\u2010\u03b1\u2010induced CaCO\u20102 cells. The colon structure destroyed in DSS\u2010induced colitis mice was improved by CP\u201025. CP\u201025 has a role in inhibition membrane translocation of GRK2\u2010\u03b2\u2010arrestin 2 complex, stabilization of the binding of GRK2 and \u03b2\u2010arrestin 2 to ERK1\/2 in cytoplasm. Subsequently down\u2010regulated the nuclear transcription and transactivation of NF\u2010\u03baB p65 via inhibiting its phosphorylation of Ser536, and Ser276, respectively and restored the epithelial barrier function. In conclusion, CP\u201025 inhibited ERK1\/2\u2010NF\u2010\u03baB activation and thereby protected the intestinal epithelial barrier, which was associated with restoring the inhibition of GRK2 and \u03b2\u2010arrestin 2 on ERK1\/2.<\/jats:p>","DOI":"10.1002\/ptr.7657","type":"journal-article","created":{"date-parts":[[2022,10,12]],"date-time":"2022-10-12T16:16:11Z","timestamp":1665591371000},"page":"743-758","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Paeoniflorin\u20106\u2032\u2010O\u2010benzene sulfonate protected the intestinal epithelial barrier by restoring the inhibitory effect of <scp>GRK2<\/scp> and \u03b2\u2010arrestin 2 on <scp>ERK1<\/scp>\/<scp>2\u2010NF\u2010\u03baB<\/scp>"],"prefix":"10.1002","volume":"37","author":[{"given":"Mengya","family":"Jiang","sequence":"first","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caihong","family":"Tang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wu","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3117-9598","authenticated-orcid":false,"given":"Wei","family":"Wei","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9421-9577","authenticated-orcid":false,"given":"Jingyu","family":"Chen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Anhui Medical University, Key Laboratory of Anti\u2010inflammatory and Immune Medicine, Ministry of Education Anhui Collaborative Innovation Center of Anti\u2010inflammatory and Immune Medicine  Hefei People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.12.016"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2012.01921.x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep26239"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00563"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.25.18.7966-7975.2005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2020.106199"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0092-8674(02)00703-1"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M611526200"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10400"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026619666181130140350"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2021.01.015"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs20191032"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgh198"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.05.034"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-018-0196-2"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e05-05-0399"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800945200"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-030409-101225"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409532102"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2008.00385.x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.5217\/ir.2015.13.1.11"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.308"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1700909114"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00173.2003"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.6336"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes4020101"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-13-9367-9_10"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3661"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2012-302830"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aal3395"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22384"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004393"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2006.03.008"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2006.09.019"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.43.30353"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21403"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-018-0169-5"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.2MA1214-599R"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00006.2018"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1042\/bcj20170093"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0136311"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.00057"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.Can-05-2957"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.03.164"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0757-z"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-020-0453-0"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06005"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-020-00510-6"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7657","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ptr.7657","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.7657","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T09:29:03Z","timestamp":1692350943000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.7657"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,12]]},"references-count":48,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2023,2]]}},"alternative-id":["10.1002\/ptr.7657"],"URL":"https:\/\/doi.org\/10.1002\/ptr.7657","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,12]]},"assertion":[{"value":"2021-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-10-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}