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In this study, RNA sequencing (RNA\u2010seq) was applied to dissect the effect of pectolinarigenin on the transcriptome changes in the high lipid Huh\u20107 cells induced by oleic acid. RNA\u2010seq results revealed that 15 pathways enriched by downregulated genes are associated with cell metabolism including cholesterol metabolism, glycerophospholipid metabolism, steroid biosynthesis, steroid hormone biosynthesis, fatty acid biosynthesis, etc. Moreover, 13 key genes related to lipid metabolism were selected. Among them, PPARG coactivator 1 beta (PPARGC1B) and carnitine palmitoyltransferase 1A (CPT1A) were found to be upregulated, solute carrier family 27 member 1(SLC27A1), acetyl\u2010CoA carboxylase alpha (ACACA), fatty\u2010acid synthase (FASN), 3\u2010Hydroxy\u20103\u2010Methylglutaryl\u2010CoA Reductase (HMGCR), etc. were found to be downregulated. Glycolysis\/gluconeogenesis, steroid hormone biosynthesis, and fatty acid biosynthesis were all significantly downregulated, according to gene set variation analysis and gene set enrichment analysis. Besides, protein levels of FASN, ACACA, and SLC27A1 were all decreased, whereas PPAR\u03b3 and CPT1A were increased. Docking models showed that PPAR\u03b3 may be a target for pectolinarigenin. Furthermore, pectolinarigenin reduced serum TG and hepatic TG, and improved insulin sensitivity in vivo. Our findings suggest that pectolinarigenin may target PPAR\u03b3 and prevent fatty acid biosynthesis.<\/jats:p>","DOI":"10.1002\/ptr.7679","type":"journal-article","created":{"date-parts":[[2022,11,23]],"date-time":"2022-11-23T06:52:52Z","timestamp":1669186372000},"page":"913-925","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Pectolinarigenin shows lipid\u2010lowering effects by inhibiting fatty acid biosynthesis in vitro and in vivo"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7210-4181","authenticated-orcid":false,"given":"Zhou","family":"Lan","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Drug Design and Evaluation, Jiangxi Key Laboratory of Organic Chemistry Jiangxi Science and Technology Normal University  Nanchang 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