{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T22:25:51Z","timestamp":1793139951976,"version":"4.5.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T00:00:00Z","timestamp":1696809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104880"],"award-info":[{"award-number":["82104880"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","award":["202308110213"],"award-info":[{"award-number":["202308110213"]}],"id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ursolic acid (UA) is a naturally occurring pentacyclic triterpenoid widely found in fruits and vegetables. It has been reported that UA has anti\u2010inflammatory effects. However, its efficacy and mechanism of action in the treatment of chronic prostatitis (CP) remain unclear. This study aimed to investigate the efficacy of UA treatment in CP and further explore the underlying mechanism. CP rat and pyroptosis cell models were established in vivo and in vitro, respectively. The efficacy of UA in inhibiting CP was evaluated via haematoxylin\u2010eosin (HE) staining and measurement of inflammatory cytokines. RNA sequencing and molecular docking were used to predict the therapeutic targets of UA in CP. The expression of pyroptosis\u2010related proteins was examined using various techniques, including immunohistochemistry, immunofluorescence, and flow cytometry. UA significantly ameliorated pathological damage and reduced the levels of proinflammatory cytokines in the CP model rats. RNA sequencing analysis and molecular docking suggested that NLRP3, Caspase\u20101, and GSDMD may be key targets. We also found that UA decreased ROS levels, alleviated oxidative stress, and inhibited p\u2010NF\u2010\u03baB protein expression both in vivo and in vitro. UA improved pyroptosis morphology as indicated by electron microscope and inhibited the expression of the pyroptosis\u2010related proteins NLRP3, Caspase\u20101, ASC, and GSDMD, reversed the levels of IL\u20101\u03b2, IL\u201018, and lactate dehydrogenase in vivo and in vitro\n                    <jats:italic>.<\/jats:italic>\n                    UA can mitigate CP by regulating the NLRP3 inflammasome\u2010mediated Caspase\u20101\/GSDMD pathway. Therefore, UA may be a potential for the treatment of CP.\n                  <\/jats:p>","DOI":"10.1002\/ptr.8034","type":"journal-article","created":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:05:57Z","timestamp":1697058357000},"page":"82-97","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Ursolic acid alleviates chronic prostatitis via regulating\n                    <scp>NLRP3<\/scp>\n                    inflammasome\u2010mediated Caspase\u20101\/\n                    <scp>GSDMD<\/scp>\n                    pyroptosis pathway"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2828-0959","authenticated-orcid":false,"given":"Sheng\u2010jing","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo\u2010da","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing\u2010he","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010jun","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Andrology Beijing Chinese Medicine Hospital affiliated to Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Andrology Xiyuan Hospital of China Academy of Chinese Medical Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,10,9]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12610"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4213"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22580"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.24214"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2016.100"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathmechdis-032521-102529"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature18590"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.45939"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2020.8257"},{"key":"e_1_2_11_11_1","article-title":"Non\u2010pharmacological interventions for treating chronic prostatitis\/chronic pelvic pain syndrome","volume":"5","author":"Franco J. 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