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Therapeutic angiogenesis regulation offers promising clinical implications, and natural compounds as pro\u2010angiogenic nutraceuticals hold valuable applications in regenerative medicine. By using cultured endothelial cells from human umbilical veins (HUVEC) we studied functional and molecular responses following exposure to erucin, a natural isothiocyanate derived from Brassicaceae plants and extracted from the seeds of rocket. Erucin (at nanomolar concentrations) promotes cell migration and tube formation, similar to vascular endothelial growth factor (VEGF), through mobilizing paxillin at endothelial edges. At the molecular level, erucin induces signaling pathways typical of angiogenesis activation, namely Ras, PI3K\/AKT, and ERK1\/2, leading to VEGF expression and triggering its autocrine production, as pharmacological inhibition of soluble VEGF and VEGFR2 dampens endothelial functions. Furthermore, erucin, alone and together with VEGF, preserves endothelial angiogenic functions under pathological conditions, such as those induced in HUVEC by high glucose (HG) exposure. Erucin emerges as a compelling candidate for therapeutic revascularization applications, showcasing promising prospects for natural compounds in regenerative medicine, particularly in addressing angiogenesis\u2010related disorders.<\/jats:p>","DOI":"10.1002\/ptr.8183","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T06:24:44Z","timestamp":1710570284000},"page":"2641-2655","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Erucin, a natural isothiocyanate, exerts pro\u2010angiogenic properties in cultured endothelial cells and reverts angiogenic impairment induced by high glucose"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7552-5085","authenticated-orcid":false,"given":"Shirley","family":"Genah","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Oncology, Bambino Ges\u00f9 Children's Hospital IRCCS  Rome 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