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The study utilized techniques such as network pharmacology, transcriptome sequencing to investigate the mechanism and target of salidroside in regulating blood\u2013brain barrier (BBB) function to protect hypoxic brain injury in vivo. Utilized macromolecular docking and molecular biology techniques to explore the molecular mechanism of salidroside in alleviating brain injury induced by hypoxia in BV2 cell model. The results show that salidroside alleviated the learning and memory dysfunction and pathological injury in mice exposed to hypobaric hypoxia, reduced brain water content and attenuate the inflammatory response and oxidative stress, effectively reversed S100\u03b2 in serum and promoted the repair of BBB. GSK3\u03b2 is an important therapeutic target of salidroside in the treatment of hypoxic cognitive impairment, and salidroside can specifically bind GSK3\u03b2 in the ATP binding pocket, inducing the phosphorylation of GSK3\u03b2, targeting downstream Nrf\u20102 to regulate microglia activity, promoting the accumulation of \u03b2\u2010catenin, thereby inhibiting microglial activation, improving the BBB integrity injury and achieving a neuroprotective effect. This study demonstrates that salidroside can inhibit the activation of microglia by inducing GSK3\u03b2 phosphorylation, achieve neuroprotective effects and alleviate learning and memory dysfunction in hypobaric hypoxia mice. This study provides a theoretical basis for the development of salidroside and the clinical application of <jats:italic>Rhodiola rosea<\/jats:italic> L.<\/jats:p>","DOI":"10.1002\/ptr.8264","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T08:58:56Z","timestamp":1728032336000},"page":"1808-1825","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Salidroside improves <scp>blood\u2013brain barrier<\/scp> integrity and cognitive function in hypobaric hypoxia mice by inhibiting microglia activation through <scp>GSK3\u03b2<\/scp>"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3166-3694","authenticated-orcid":false,"given":"Xianxie","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences Beijing Institute of Radiation Medicine  Beijing People's Republic of 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