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Different reports demonstrated biological activities of aged black garlic (ABG), including anti\u2010inflammatory and antioxidant effects. We aimed to investigate beneficial effects exerted by ABGE on colon inflammation by using ex vivo and in vivo experimental models. We investigated the anti\u2010inflammatory effects of an ABG water extract (ABGE) on rat colon specimens exposed to <jats:italic>E. coli<\/jats:italic> lipopolysaccharide (LPS), a known ex vivo experimental model of ulcerative colitis. We determined gene expression of various biomarkers involved in inflammation, including interleukin (IL)\u20101\u03b2, IL\u20106, nuclear factor\u2010kB (NF\u2010kB), tumor necrosis factor (TNF)\u2010\u03b1. Moreover, we studied the acute effects of ABGE on visceral pain associated with colitis induced by 2,4\u2010di\u2010nitrobenzene sulfonic acid (DNBS) injection in rats. ABGE suppressed LPS\u2010induced gene expression of IL\u20101\u03b2, IL\u20106, NF\u2010kB, and TNF\u2010\u03b1. In addition, the acute administration of ABGE (0.03\u20131\u2009g\u2009kg<jats:sup>\u22121<\/jats:sup>) dose\u2010dependently relieved post\u2010inflammatory visceral pain, with the higher dose (1\u2009g\u2009kg<jats:sup>\u22121<\/jats:sup>) able to significantly reduce both the behavioral nociceptive response and the entity of abdominal contraction (assessed by electromyography) in response to colorectal distension after the acute administration in DNBS\u2010treated rats. Present findings showed that ABGE could represent a potential strategy for treatment of colitis\u2010associated inflammatory process and visceral pain. The beneficial effects induced by the extract could be related to the pattern of polyphenolic composition, with particular regard to gallic acid and catechin.<\/jats:p>","DOI":"10.1002\/ptr.8270","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T09:04:26Z","timestamp":1719219866000},"page":"4177-4188","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Anti\u2010inflammatory and anti\u2010hyperalgesic effects induced by an aqueous aged black garlic extract in rodent models of ulcerative colitis and colitis\u2010associated visceral pain"],"prefix":"10.1002","volume":"38","author":[{"given":"Maria Loreta","family":"Libero","sequence":"first","affiliation":[{"name":"Department of Pharmacy \u201cG. d'Annunzio\u201d University  Chieti Italy"},{"name":"Department of Cell Biology, Physiology and Immunology University of Cordoba  Cordoba 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