{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:58:56Z","timestamp":1783472336014,"version":"3.55.0"},"reference-count":74,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T00:00:00Z","timestamp":1723161600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Obesity is a major source of morbidity worldwide with more than 2 billion adults being overweight or obese. The incidence of obesity has tripled in the last 50\u2009years, leading to an increased risk for a variety of noncommunicable diseases. Previous studies have demonstrated the positive effects of green leafy vegetables on weight gain and obesity and have attributed these beneficial properties, at least in part, to nitrates and isothiocyanates. Nitrates are converted to nitric oxide (NO) and isothiocyanates are known to release hydrogen sulfide (H<jats:sub>2<\/jats:sub>S). Herein, we investigated the effect of extracts and fractions produced from <jats:italic>Beta vulgaris<\/jats:italic> and <jats:italic>Eruca sativa<\/jats:italic> for their ability to limit lipid accumulation, regulate glucose homeostasis, and reduce body weight. Extracts from the different vegetables were screened for their ability to limit lipid accumulation in adipocytes and hepatocytes and for their ability to promote glucose uptake in skeletal muscle cultures; the most effective extracts were next tested in vivo. Wild type mice were placed on high\u2010fat diet for 8\u2009weeks to promote weight gain; animals receiving the selected <jats:italic>B. vulgaris<\/jats:italic> and <jats:italic>E. sativa<\/jats:italic> extracts exhibited attenuated body weight. Treatment with extracts also led to reduced white adipose tissue depot mass, attenuated adipocyte size, reduced expression of <jats:italic>Dgat2<\/jats:italic> and <jats:italic>PPAR\u03b3<\/jats:italic> expression, and improved liver steatosis. In contrast, the extracts failed to improve glucose tolerance in obese animals and did not affect blood pressure. Taken together, our data indicate that extracts produced from <jats:italic>B. vulgaris<\/jats:italic> and <jats:italic>E. sativa<\/jats:italic> exhibit anti\u2010obesity effects, suggesting that dietary supplements containing nitrates and sulfide\u2010releasing compounds might be useful in limiting weight gain.<\/jats:p>","DOI":"10.1002\/ptr.8291","type":"journal-article","created":{"date-parts":[[2024,8,9]],"date-time":"2024-08-09T13:15:44Z","timestamp":1723209344000},"page":"4757-4773","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Anti\u2010obesity effects of <i>Beta vulgaris<\/i> and <i>Eruca sativa<\/i>\u2010based extracts"],"prefix":"10.1002","volume":"38","author":[{"given":"Maria","family":"Markou","sequence":"first","affiliation":[{"name":"Center of Clinical, Experimental Surgery &amp; Translational Research Biomedical Research Foundation of the Academy of Athens  Athens Greece"},{"name":"Laboratory of Pharmacology, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Katsouda","sequence":"additional","affiliation":[{"name":"Center of Clinical, Experimental Surgery &amp; Translational Research Biomedical Research Foundation of the Academy of Athens  Athens Greece"},{"name":"Laboratory of Pharmacology, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Varvara","family":"Papaioannou","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aikaterini","family":"Argyropoulou","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"},{"name":"PharmaGnose S.A.  Oinofyta Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianna","family":"Vanioti","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constantin","family":"Tamvakopoulos","sequence":"additional","affiliation":[{"name":"Center of Clinical, Experimental Surgery &amp; Translational Research Biomedical Research Foundation of the Academy of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandros A.","family":"Skaltsounis","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Halabalaki","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Mitakou","sequence":"additional","affiliation":[{"name":"Division of Pharmacognosy and Natural Products Chemistry, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4253-5930","authenticated-orcid":false,"given":"Andreas","family":"Papapetropoulos","sequence":"additional","affiliation":[{"name":"Center of Clinical, Experimental Surgery &amp; Translational Research Biomedical Research Foundation of the Academy of Athens  Athens Greece"},{"name":"Laboratory of Pharmacology, Department of Pharmacy National and Kapodistrian University of Athens  Athens Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1614362"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/hr.2014.125"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13679-020-00398-7"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.12.010"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu8120811"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfca.2016.06.006"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/oby.23110"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2022.115381"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705710104"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/mnfr.201700478"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.118.034757"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-789X.2008.00503.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.109.920991"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008872107"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00028.2021"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241511886"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1809406115"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0695-546"},{"key":"e_1_2_9_20_1","first-page":"1","article-title":"Nitrate in vegetables: Scientific opinion of the Panel on Contaminants in the Food Chain","volume":"689","author":"EFSA","year":"2008","journal-title":"The EFSA Journal"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.19897"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705891104"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2013.33222"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200303"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-018-0427-3"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30752-2"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2017.08.003"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2019.08.006"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.01.024"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.34"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1161\/hypertensionaha.118.12425"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1514009"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.048"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2009.03.005"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2013.10.809"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci129187"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1161\/hypertensionaha.114.04675"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20211894"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2014.11.011"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.12.012"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-015-0872-7"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3402\/fnr.v60.32010"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.9b02117"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.06.007"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.260"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.06.010"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd2466"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.14645"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9111038"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-021-00337-8"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.06.003"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101387"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6941"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2018.11.014"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.2337\/db14-0496"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/nt.0000000000000228"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2016.04.016"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.13176"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.05.010"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105121108"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2011.4451"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1124\/pr.117.014050"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/53.1.79"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.numecd.2021.04.011"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00525.2004"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcme.2014.11.033"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.3945\/ajcn.115.116244"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.02.017"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1161\/atvbaha.108.179333"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13679-015-0169-4"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00365.2002"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1089\/ars.2012.5024"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2003.11.081"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1162667"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2012.02.028"}],"container-title":["Phytotherapy Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ptr.8291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T13:49:05Z","timestamp":1729691345000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ptr.8291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,9]]},"references-count":74,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2024,9]]}},"alternative-id":["10.1002\/ptr.8291"],"URL":"https:\/\/doi.org\/10.1002\/ptr.8291","archive":["Portico"],"relation":{},"ISSN":["0951-418X","1099-1573"],"issn-type":[{"value":"0951-418X","type":"print"},{"value":"1099-1573","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,9]]},"assertion":[{"value":"2024-02-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}