{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T20:10:52Z","timestamp":1793045452047,"version":"4.5.0"},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T00:00:00Z","timestamp":1736467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100014824","name":"Department of Pharmaceuticals, Ministry of Chemicals and Fertilizers, India","doi-asserted-by":"publisher","award":["Institutional Grant through NIPER Raebareli"],"award-info":[{"award-number":["Institutional Grant through NIPER Raebareli"]}],"id":[{"id":"10.13039\/501100014824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Background and aim: Hepatic encephalopathy (HE) is a complex neurological disorder in individuals with liver diseases, necessitating effective neuroprotective interventions to alleviate its adverse outcomes. Berberine (BBR), a natural compound with well\u2010established anti\u2010fibrotic and neuroprotective properties, has not been extensively studied in the context of glial activation under hyperammonaemic conditions. This study evaluates the neuroprotective potential of BBR in a thioacetamide (TAA)\u2010induced HE rat model, focusing on its effects on glial activation and NLRP3 inflammasome signalling. Methods: Neurological impairments were assessed using open field tests and sensory analysis. Western blotting was performed to evaluate the expression of glial and neuronal markers, tight junction proteins and NLRP3 inflammasome components in the cortex and hippocampus. Histopathological and molecular changes were further examined using H&amp;E, immunohistochemistry and immunofluorescence staining. Key Results: BBR treatment significantly improved behavioural abnormalities and reduced systemic ammonia levels in TAA\u2010exposed rats. It restored blood\u2013brain barrier integrity, as evidenced by reduced tight junction protein degradation. BBR inhibited the expression of NLRP3 inflammasome markers, including caspase\u20101, IL\u20101\u03b2, ASC, and NF\u2010\u03baB, while reducing glial cell activation (IBA\u20101 and GFAP). Notably, BBR diminished NLRP3 expression in glial cells, indicating its potent anti\u2010inflammatory effects. Additionally, BBR preserved neuronal integrity, as demonstrated by the maintained expression of MAP\u20102 and NeuN and reduced cleaved Gasdermin D levels. Conclusions: These findings suggest that BBR alleviates behavioural and molecular abnormalities in HE through NLRP3 inflammasome inhibition, highlighting its potential as a therapeutic agent for managing HE.<\/jats:p>","DOI":"10.1002\/ptr.8430","type":"journal-article","created":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T08:09:00Z","timestamp":1736496540000},"page":"1422-1437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Berberine Inhibits the Disruption of the Blood\u2013Brain Barrier and Glial Cell Activation in a Rat Model of Acute Hepatic Encephalopathy"],"prefix":"10.1002","volume":"39","author":[{"given":"Syed Afroz","family":"Ali","sequence":"first","affiliation":[{"name":"Laboratory of Molecular NeuroTherapeutics, Department of Pharmacology and Toxicology National Institute of Pharmaceutical Education and Research  Raebareli Uttar Pradesh 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