{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T12:30:33Z","timestamp":1792499433732,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD1600200"],"award-info":[{"award-number":["2023YFD1600200"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Phytotherapy Research"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Inflammatory bowel disease (IBD) is a global health concern with limited therapeutic options. Previous studies have demonstrated that piperine exhibited anti\u2010inflammatory effects both in\u00a0vitro and in\u00a0vivo. However, its potential to ameliorate colitis in mice through modulation of gut microbiota has not been explored. This study aimed to investigate the role of gut microbiota in the protective effects of piperine against colitis using a dextran sulfate sodium (DSS)\u2010induced mouse model. Mice were administered piperine (12.5 and 25\u2009mg\/kg) prior to DSS exposure. Fecal microbiota transplantation (FMT) was then performed, after which we evaluated colitis symptoms, inflammation levels, and intestinal barrier function. Subsequently, 16S rDNA\u2010based high\u2010throughput sequencing was employed to analyze the microbial composition of the mouse cecal contents. Piperine administration increased the colon length, decreased the spleen index, and improved colon histopathology. Furthermore, piperine modulated inflammatory responses by inhibiting NF\u2010\u03baB signaling, thereby reducing the release of pro\u2010inflammatory cytokines and mediators. It also enhanced intestinal barrier integrity by increasing the expression of claudin\u20101, claudin\u20103, ZO\u20101, occludin, and mucin 2. Notably, the 16S rDNA sequencing results revealed that piperine increased the abundance of\n                    <jats:italic>Dubosiella<\/jats:italic>\n                    in the gut. Piperine effectively protected mice from DSS\u2010induced colitis, suppressed inflammation, and improved poor intestinal barrier function. It reshaped the intestinal microbiota, ultimately alleviating DSS\u2010induced colitis in mice. Our research highlighted the significant role of gut microbiota in the piperine\u2010mediated alleviation of intestinal damage and suggested its therapeutic potential for promoting gut health and reducing the risk of colitis.\n                  <\/jats:p>","DOI":"10.1002\/ptr.8491","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T20:23:40Z","timestamp":1748895820000},"page":"3197-3211","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Piperine Improves\n                    <scp>DSS<\/scp>\n                    \u2010Induced Colitis in Mice via Inhibition of Inflammation and Modulation of Gut Microbiota"],"prefix":"10.1002","volume":"39","author":[{"given":"Yeye","family":"Hu","sequence":"first","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"},{"name":"The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid &amp; Cell Fate Regulation Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanting","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyang","family":"Gao","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guigui","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziliang","family":"He","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghui","family":"Lv","sequence":"additional","affiliation":[{"name":"Hainan Hospital of Guangdong Provincial Hospital of Traditional Chinese Medicine  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenglin","family":"Gu","sequence":"additional","affiliation":[{"name":"Spice and Beverage Research Institute\/Key Laboratory of Processing Suitability and Quality Control of the Special Tropical Crops of Hainan Province Chinese Academy of Tropical Agricultural Sciences  Wanning Hainan China"},{"name":"Sanya Research Institute of Chinese Academy of Tropical Agricultural Sciences  Sanya Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaoqing","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Jinan University  Guangzhou China"},{"name":"Zhejiang Weikang Pharmaceutical Co., Ltd.  Lishui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6730-811X","authenticated-orcid":false,"given":"Weicheng","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Translational Medicine, School of Medicine Yangzhou University  Yangzhou China"},{"name":"The Key Laboratory of the Jiangsu Higher Education Institutions for Nucleic Acid &amp; Cell Fate Regulation Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00616\u201022"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.22159\/ajpcr.2022v15i11.45703"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2010.05.026"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl\u20102020\u2010321106"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2022.109545"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14224744"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2023.09.019"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0960327119892042"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.01.039"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6855"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2022.08.018"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/life12040476"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2015.04.006"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2023.105499"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155024"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000653.v3"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2012.04.024"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112452"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168\u2010019\u20100725\u20103"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0000000000001396"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom14070873"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753\u2010012\u20109448\u20103"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140\u20106736(17)32448\u20100"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.733992"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575\u2010023\u201000854\u20104"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010020\u201015342\u20105"},{"key":"e_1_2_12_28_1","first-page":"217","article-title":"Study of Antidiarrhoeal Activity of Piperine","volume":"4","author":"Shamkuwar P. 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