{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T10:08:35Z","timestamp":1753870115375,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T00:00:00Z","timestamp":1742774400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Public Health Challenges"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Interaction among humans, livestock, and wildlife plays an important role in zoonotic disease transmission. The emergence of <jats:italic>Leptospira<\/jats:italic> in humans, rodents, and cattle remains relatively understudied. A cross\u2010sectional study was conducted between February and May 2023 in Muheza to determine evidence of <jats:italic>Leptospira<\/jats:italic> infection and associated factors in rodents, cattle, and humans. A total of 479 serum samples from rodents (<jats:italic>n<\/jats:italic>\u00a0=\u00a0201), humans (<jats:italic>n<\/jats:italic>\u00a0=\u00a0198), and cattle (<jats:italic>n<\/jats:italic>\u00a0=\u00a080) were examined by microscopic agglutination test (MAT) to detect antibodies against 6 live <jats:italic>Leptospira<\/jats:italic> stock culture serovars, including Pomona, Hebdomadis, Canicola, Grippotyphosa, Sokoine, and Lora. Additionally, a questionnaire survey was conducted on 140 respondents to determine factors that are associated with <jats:italic>Leptospira<\/jats:italic> seropositivity. Descriptive statistics and Chi\u2010square test were used to analyze the data. The overall <jats:italic>Leptospira<\/jats:italic> seroprevalence in rodents, cattle, and humans was 6.0% (12\/201; 95% CI: 3.12%\u201310.20%), 12.5% (10\/80; 95% CI: 6.16%\u201321.79%), and 13.1% (26\/198; 95% CI: 8.76%\u201318.65%), respectively, and the most predominant serovars were Grippotyphosa, Sokoine, and Hebdomadis. A significant difference in the seroprevalence was observed in occupation, whereby farmers were more likely to be infected with <jats:italic>Leptospira<\/jats:italic> than those in other occupations (<jats:italic>\u03c7<\/jats:italic><jats:sup>2\u00a0<\/jats:sup>=\u00a09.19, df\u00a0=\u00a03, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.027). This study showed co\u2010agglutination among rodents, cattle, and humans with serovars Hebdomadis, Sokoine, and Grippotyphosa. People aged 36\u201359 had the highest seropositivity, suggesting they are the most at\u2010risk group. This study shed light on pathogenic serovars circulating among humans, rodents, and cattle and factors associated with seropositivity. The findings appeal for multisectoral One Health approach for effective control of <jats:italic>Leptospira<\/jats:italic> infection and other zoonotic diseases.<\/jats:p>","DOI":"10.1002\/puh2.70043","type":"journal-article","created":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T23:27:56Z","timestamp":1742945276000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Detection and Determinants of <i>Leptospira<\/i> Infection in Rodents, Cattle, and Humans in Muheza District, Tanzania: A Call for One Health Approach"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-3331-0244","authenticated-orcid":false,"given":"Gamba Gerald","family":"Manyama","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine and Public Health Sokoine University of Agriculture  Morogoro Tanzania"},{"name":"African Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACEII, IRPM\u2010BTD) of the Sokoine University of Agriculture  Morogoro Tanzania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald Dickson","family":"Mlowe","sequence":"additional","affiliation":[{"name":"Department of Animal, Aquaculture and Range Sciences Sokoine University of Agriculture  Morogoro Tanzania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athumani Msalale","family":"Lupindu","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine and Public Health Sokoine University of Agriculture  Morogoro Tanzania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdul Suleman","family":"Katakweba","sequence":"additional","affiliation":[{"name":"African Centre of Excellence for Innovative Rodent Pest Management and Biosensor Technology Development (ACEII, IRPM\u2010BTD) of the Sokoine University of Agriculture  Morogoro Tanzania"},{"name":"Institute of Pest Management Sokoine University of Agriculture  Morogoro Tanzania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003899"},{"key":"e_1_2_9_3_1","first-page":"195","article-title":"Laboratory Diagnosis of Leptospirosis","volume":"51","author":"Ahmad S. 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