{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T05:39:09Z","timestamp":1766986749930,"version":"3.48.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T00:00:00Z","timestamp":1761350400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Public Health Challenges"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    is a zoonotic protozoan that poses serious threats to public health through contaminated animal products. The frequent interactions between inhabitants in the Kassena\u2013Nankana districts and livestock increase the risk of zoonotic infections. However, there is limited information on the circulation of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    within the districts. This study aimed to determine the prevalence of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    infection in livestock species within the Kassena\u2013Nankana districts using a PCR\u2010based diagnostic technique. Dry blood spots collected from February to December 2020 by convenience sampling were screened using PCR amplification of a 529\u00a0bp repetitive fragment specific to\n                    <jats:italic>T. gondii<\/jats:italic>\n                    . A total of 374 samples were collected from cattle (26.74%), chicken (26.20%), sheep (25.40%) and goats (21.66%). The overall prevalence of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    was 13.9%, with all positives being from chicken samples. No infections were detected in the sampled cattle, sheep or goats. Free\u2010range chickens were more likely to be infected with\n                    <jats:italic>T. gondii<\/jats:italic>\n                    (odds ratios [OR]\u00a0=\u00a02, 95% confidence interval [CI]\u00a0=\u00a00.43\u20139.88,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.38). Furthermore, it was observed that chicken from Namolo had a lower risk of infections (OR\u00a0=\u00a00.11, 95% CI: 0.02\u20130.43,\n                    <jats:italic>p<\/jats:italic>\n                    \u00a0=\u00a00.006). To reduce the dangers to public health, frequent education, improved poultry management and continuous surveillance within the districts are advised.\n                  <\/jats:p>","DOI":"10.1002\/puh2.70159","type":"journal-article","created":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T10:41:13Z","timestamp":1761388873000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection of\n                    <i>Toxoplasma gondii<\/i>\n                    in Chickens and Ruminants: A Study From Kassena\u2010Nankana Districts, Ghana"],"prefix":"10.1002","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4470-4504","authenticated-orcid":false,"given":"Seth Offei","family":"Addo","sequence":"first","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Margaret","family":"Addo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology University of Ghana Medical School University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher Nii Laryea","family":"Tawiah\u2010Mensah","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel Kwame","family":"Amoako","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"},{"name":"Immunology Department, Noguchi Memorial Institute for Medical Research  University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer Nyamekye","family":"Yanney","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisca Adai","family":"Torto","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard Odoi\u2010Teye","family":"Malm","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacy","family":"Amoah","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4105-1010","authenticated-orcid":false,"given":"Samuel K.","family":"Dadzie","sequence":"additional","affiliation":[{"name":"Parasitology Department, Noguchi Memorial Institute for Medical Research University of Ghana  Accra Ghana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,25]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20103305\u20103\u2010112"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013\u201011"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2471\/blt.12.111732"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.VETPAR.2015.09.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.VETPAR.2011.09.012"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.VETPAR.2019.06.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182015000050"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00436\u2010017\u20105730\u20105\/METRICS"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1556\/EUJMI\u2010D\u201014\u201000024"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12879\u2010021\u201006658\u20108\/TABLES\/2"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2024.2396577"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/VMS3.1255"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/FVETS.2018.00014\/PDF"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2018.04.4110"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001\u2010706X(00)00085\u20101"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0001\u2010706X(00)00084\u2010X"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13071\u2010015\u20100902\u20106"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.160"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00170-8"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010017\u20105442\u2010x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15321819.2019.1642916"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14753"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE\u20101362.1"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182020001134"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0232026"},{"issue":"6","key":"e_1_2_10_27_1","first-page":"1705","article-title":"Seroprevalence of Toxoplasma gondii Infection in Gallus domesticus of District Mardan, Khyber Pakhtunkhwa, Pakistan","volume":"46","author":"Mahmood Z. U.","year":"2014","journal-title":"Pakistan Journal of Zoology"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/PUH2.82"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0324950"},{"issue":"3","key":"e_1_2_10_30_1","first-page":"107","article-title":"Sero\u2010Epidemiology of Toxoplasmosis amongst Pregnant Women in the Greater Accra Region of Ghana","volume":"43","author":"Ayi I.","year":"2009","journal-title":"Ghana Medical Journal"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s41182\u2010016\u20100018\u20105"}],"container-title":["Public Health Challenges"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/puh2.70159","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T05:34:28Z","timestamp":1766986468000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/puh2.70159"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,25]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/puh2.70159"],"URL":"https:\/\/doi.org\/10.1002\/puh2.70159","archive":["Portico"],"relation":{},"ISSN":["2769-2450","2769-2450"],"issn-type":[{"type":"print","value":"2769-2450"},{"type":"electronic","value":"2769-2450"}],"subject":[],"published":{"date-parts":[[2025,10,25]]},"assertion":[{"value":"2025-06-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70159"}}