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However, its potential protective effect and mechanism of action in acute lung injury (ALI) have not been explored. We aimed to explore the mechanisms underlying the protective role of QKPCG in ALI. The therapeutic efficacy of QKPCG was investigated in a lipopolysaccharide (LPS)\u2010induced ALI mouse model. Mice were divided into three groups, namely, the Control, LPS, and LPS\u2009+\u2009QKPCG groups. Mice in the LPS\u2009+\u2009QKPCG group were administered QKPCG intragastrically as a treatment once a day for a total of three days. QKPCG effectively increased survival and reduced lung injury in treated mice. It significantly reduced the LPS\u2010induced expression of interleukin\u00a0(IL)\u20106, tumor necrosis factor\u2010\u03b1\u00a0(TNF\u2010\u03b1), IL\u20101\u03b1, and IL\u20101\u03b2. RNA\u2010sequencing followed by real\u2010time quantitative polymerase chain reaction\u00a0validation suggested a critical role of the secretoglobin family 1A member 1 (<jats:italic>Scgb1a1<\/jats:italic>) gene in mediating the protective effect of QKPCG. Further, QKPCG reversed the LPS\u2010induced downregulation of the Clara cell 10\u2009kDa protein (CC10), a pulmonary surfactant protein encoded by <jats:italic>Scgb1a1<\/jats:italic>, which is mainly secreted by club cells in the lungs. Exogenous supplementation of CC10 alleviated LPS\u2010induced ALI. Hematoxylin and eosin\u00a0staining and enzyme\u2010linked immunosorbent assay\u00a0results further confirmed the anti\u2010inflammatory properties of CC10, which were suggested as mediated via the inhibition of NF\u03baB phosphorylation. In summary, our study provides evidence of the beneficial role of QKPCG in alleviating lung injury, mediated via the decreased disruption of club cells and higher expression of CC10, which leads to NF\u03baB pathway inhibition.<\/jats:p>","DOI":"10.1002\/pul2.12138","type":"journal-article","created":{"date-parts":[[2022,9,15]],"date-time":"2022-09-15T14:54:55Z","timestamp":1663253695000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The traditional Chinese patented medicine Qingke Pingchuan granules alleviate acute lung injury by regenerating club cells"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6262-1513","authenticated-orcid":false,"given":"Yuanyuan","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Pulmonary and Critical Care Medicine, Shanghai Respiratory Research Institute, Zhongshan Hospital Fudan University  Shanghai China"},{"name":"Shanghai 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