{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:51Z","timestamp":1772118951557,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T00:00:00Z","timestamp":1746489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Tongfu Xiexia Decoction (TFXXD), a traditional Chinese medicine formula, comprises six herbs: semen raphani, Rehmannia glutinosa, rhubarb,\n                      <jats:italic>Magnolia officinalis<\/jats:italic>\n                      , trifoliate orange, and mirabilite. The aim of this study was to investigate the effects of TFXXD on its biotransformation and microbial abundance in the gut of constipated rats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Ultrahigh\u2010performance liquid chromatography\u2010quadrupole time\u2010of\u2010flight mass spectrometry (UHPLC\u2010Q\u2010TOF\u2010MS\/MS) was used to analyze the biotransformation of TFXXD in the colonic contents, and 16S rRNA sequencing was used to assess the structure and diversity of the gut microbiota across various rat groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified and analyzed 25 biotransformations of TFXXD in the colonic contents, which undergo various reactions such as deglycosylation, ring opening, and hydration in intestinal bacteria, with 14 originating from trifoliate orange, six from rhubarb, three from Rehmannia glutinosa, one from Semen raphani, and one from\n                      <jats:italic>M. officinalis<\/jats:italic>\n                      . 16S rRNA analysis revealed that TFXXD significantly enhanced the relative abundance of beneficial bacteria, such as\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      and\n                      <jats:italic>Bacteroides<\/jats:italic>\n                      while significantly reducing\n                      <jats:italic>Oscillospira<\/jats:italic>\n                      abundance. Moreover, TFXXD considerably affected the carbohydrate and amino acid metabolism. Correlation analyses revealed a significant negative correlation between\n                      <jats:italic>Bacteroides<\/jats:italic>\n                      and ACH (Acetylcholine), NO (nitric oxide), D\u2010Lac (D\u2010Lactate), IL\u20106 (Interleukin\u20106), TNF\u2010\u03b1 (tumor necrosis factor\u2010\u03b1), and IL\u20101\u03b2 (Interleukin\u20101\u03b2) and a significant positive correlation between\n                      <jats:italic>Bacteroides<\/jats:italic>\n                      , hesperetin, and rhein.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In conclusion, our findings indicate that TFXXD can modulate the structure and diversity of the gut microbiota and enhance the metabolic balance in constipated rats.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10065","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T23:04:57Z","timestamp":1746572697000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrating Intestinal Biotransformation and Gut Microbiota to Uncover the Influence of Tongfu Xiexia Decoction in Rats With Constipation"],"prefix":"10.1002","volume":"39","author":[{"given":"Yu\u2010Han","family":"Piao","sequence":"first","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Luo","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Ren","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ao","family":"Wang","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Yun","family":"Li","sequence":"additional","affiliation":[{"name":"Jilin City Hospital of Chemical Industry  Jilin China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Jia","family":"Pan","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin\u2010Ze","family":"Li","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang\u2010Tong","family":"Li","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Wen","family":"Bao","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3513-4255","authenticated-orcid":false,"given":"Fei","family":"Zheng","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hao","family":"Yue","sequence":"additional","affiliation":[{"name":"Changchun University of Chinese Medicine  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389450124666221209123541"},{"issue":"1","key":"e_1_2_8_3_1","first-page":"1","article-title":"Clinical Practice Guideline on the Evaluation and Management of Chronic Constipation for Chinese Adults (2022 edition)","volume":"25","author":"Chinese Society of Colorectal Surgery C S o S, Chinese Medical A","year":"2022","journal-title":"Zhonghua wei Chang wai ke za zhi Chinese Journal of Gastrointestinal Surgery"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2021.112305"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.08.018"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/6733851"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00575"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/foods9081045"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111479"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14183704"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.863779"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/med.21989"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1353015"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2019.1684527"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.1048134"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2024.1362948"},{"key":"e_1_2_8_17_1","first-page":"91","article-title":"Metabolomics Study on the Therapeutic Effect of the Chinese Herb Pair Fructus Aurantii Immaturus and Rhizoma Atractylodis Macrocephalae in Constipated Rats Based on UPLC\u2010Q\/TOF\u2010MS Analysis","volume":"36","author":"Yan S.","year":"2021","journal-title":"Journal of Gastroenterology and Hepatology"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.137878"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.03.063"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9664"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3212"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116936"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2276"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq461"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3536"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3907"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.556"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867327666200713184138"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711796391589"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00706-011-0623-2"},{"key":"e_1_2_8_31_1","volume-title":"Part i: The Structure Elucidation of Some Neolignans. Part ii: Synthesis and Antimicrobial Activity of Cannithrene 1 and Notholaenic Acid: Two Stilbenoid Natural Products [M]","author":"Cheatham S. F.","year":"1984"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm-23-6"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.116.014407"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1FO01125A"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.658354"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.crfs.2024.100875"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31033-0"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00455"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2022.2098249"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0255651"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2022.154401"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1936"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.70085"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.3907"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.12.055"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2020.113056"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.107979"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14234945"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02063253"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.10065","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T09:59:28Z","timestamp":1761472768000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.10065"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,6]]},"references-count":48,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2025,8,15]]}},"alternative-id":["10.1002\/rcm.10065"],"URL":"https:\/\/doi.org\/10.1002\/rcm.10065","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.10065\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10065\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,6]]},"assertion":[{"value":"2025-02-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10065"}}