{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:44Z","timestamp":1772118944401,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,6,3]],"date-time":"2025-06-03T00:00:00Z","timestamp":1748908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The measurement of urinary catecholamines and total metanephrines serves as one of the primary tests in the diagnosis of pheochromocytoma and paraganglioma (PPGL). The widely adopted liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) method, however, typically necessitates sample pretreatments and chromatographic separation prior to MS, resulting in challenges for facile and rapid screening of targets from complex matrices. Therefore, it is crucial to develop an analytical method that can rapidly and efficiently detect urinary catecholamines and total metanephrines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rapid and time\u2010efficient procedure using solid\u2010phase extraction (SPE) combined with pulsed direct current electrospray ionization tandem mass spectrometry (pulsed\u2010dc\u2010ESI\u2010MS\/MS) was validated for the specific and quantitative analysis of six urinary catecholamines and metanephrines. The SPE protocol was specifically optimized to enable direct analysis of the eluate obtained from SPE using MS\/MS. All six compounds could be detected in a single complete operation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The method was evaluated by the determination of catecholamines and metanephrines (dopamine, epinephrine, norepinephrine, normetanephrine, metanephrine, and 3\u2010methoxytyramine) in artificial urine samples and raw urine samples. Under the optimized experimental conditions, the limits of detection for these six analytes were in the range of 0.02\u201368.37 nM\u2009L\n                      <jats:sup>\u22121<\/jats:sup>\n                      , using dopamine\u2010d4 (DA\u2010d4) and metanephrines\u2010d3 (MN\u2010d3) as internal standards, respectively, which achieved the detection requirements for the clinical diagnosis of PPGL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SPE coupled with pulsed\u2010dc\u2010ESI\u2010MS\/MS demonstrated improved efficiency compared to existing methods, which successfully enabled the rapid screening of urinary catecholamines and total metanephrines. Therefore, we believe that this method could be potentially useful in the clinical screening of PPGL and suitable for the direct analysis of urine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10077","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T01:48:51Z","timestamp":1749001731000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Solid\u2010Phase Extraction Coupled to Tandem Mass Spectrometry for Rapid Quantitation of Urinary Catecholamines and Total Metanephrines"],"prefix":"10.1002","volume":"39","author":[{"given":"Yuhan","family":"Li","sequence":"first","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui China"},{"name":"Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China  Hefei Anhui China"},{"name":"School of Chemistry and Materials Science University of Science and Technology of China  Hefei Anhui China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8391-4013","authenticated-orcid":false,"given":"Guangming","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine University of Science and Technology of China  Hefei Anhui China"},{"name":"Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China  Hefei Anhui China"},{"name":"School of Chemistry and Materials Science University of Science and Technology of China  Hefei Anhui China"},{"name":"National Synchrotron Radiation Laboratory University of Science and Technology of China  Hefei Anhui 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