{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T14:37:20Z","timestamp":1790347040706,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Clinically, cyclosporine (CsA) plays a crucial role in the treatment of aplastic anemia (AA) and has demonstrated significant therapeutic efficacy. We applied DIA\u2010based quantitative proteomics to analyze plasma protein profiles in AA patients, aiming to identify proteins and pathways modulated by CsA, thereby elucidating its therapeutic mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Plasma samples from three AA patients pre\u2013 and post\u2013CsA treatment underwent data\u2010independent acquisition proteomics. DEPs were identified using fold\u2010change thresholds. Gene Ontology, KEGG, and STRING analyses revealed functional pathways and hub proteins, which were validated by ELISA in 13 AA patients. Molecular docking assessed CsA\u2010core protein binding affinities.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared to healthy people, AA patients exhibited 303 differential proteins, enriched in pathways related to oxidative stress, cellular adhesion, and energy dysregulation. Post\u2013CsA treatment, 107 DEPs were identified, linked to redox balance and structural remodeling. Forty\u2010eight proteins overlapped, with GAPDH, SOD1, CFL1, and ACTG1 as core targets. ELISA confirmed expression differences, and molecular docking showed strong CsA binding affinities to these proteins.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Significant protein expression differences between AA patients and healthy controls suggest immune\u2010related pathways, including metabolism, oxidative stress, and cell structure, as key treatment targets. CsA may regulate these to intervene in AA progression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10085","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T09:54:29Z","timestamp":1748512469000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Mechanisms of Cyclosporine Therapy in Aplastic Anemia: A Data\u2010Independent Acquisition Proteomics Approach"],"prefix":"10.1002","volume":"39","author":[{"given":"Mingxin","family":"Guo","sequence":"first","affiliation":[{"name":"The Affiliated Yixing Clinical School of Medical School of Yangzhou University  Yixing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Dai","sequence":"additional","affiliation":[{"name":"The Affiliated Yixing Clinical School of Medical School of Yangzhou University  Yixing 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