{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,12]],"date-time":"2026-11-12T16:33:48Z","timestamp":1794501228395,"version":"4.9.1"},"reference-count":21,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,9,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Desmopressin (DDAVP), which indirectly increases Coagulation Factor VIII concentrations in the blood, is a common treatment for bleeding disorders such as von Willebrand disease or hemophilia A. However, DDAVP exhibits significant variability in response due to interindividual differences in pharmacokinetics. Consequently, exploring its pharmacokinetics is of primary importance to better understand the relationship between DDAVP administration and therapeutic outcomes. To that end, the measurement of DDAVP concentration is essential. This article describes the development and validation of a liquid chromatography method coupled with high\u2010resolution mass spectrometry detection for quantifying DDAVP in human plasma. After sample pretreatment involving protein precipitation followed by solid\u2010phase extraction, quantification was based on the four most intense isotopes, utilizing targeted single ion monitoring, with a method range of 20\u20132000\u2009pg.mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . For the four QC levels, accuracy and precision for both inter\u2010 and intra\u2010assay measurements were below 11.6% and 13.8%, respectively, meeting FDA recommendations. After validation, this method was applied to a cohort of 10 patients with a deficiency in Coagulation Factor VIII, who received 0.3\u2009\u03bcg.kg\n                    <jats:sup>\u22121<\/jats:sup>\n                    of desmopressin acetate. The mean volume of distribution at steady state was 18.8\u2009L (CV% 26.7), the mean clearance was 7.8\u2009L.h\n                    <jats:sup>\u22121<\/jats:sup>\n                    (CV% 25.1), and the mean half\u2010life was 1.8\u2009h (CV% 14.7). Offering valuable insights into the pharmacokinetics of DDAVP, this method will be useful for further studies and holds promise for optimizing treatment regimens in this patient population.\n                  <\/jats:p>","DOI":"10.1002\/rcm.10086","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T03:39:40Z","timestamp":1749526780000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Development and Validation of a Liquid Chromatography High\u2010Resolution Mass Spectrometry Method for Blood Desmopressin Quantification and Its Application in Hemophilia A Patients"],"prefix":"10.1002","volume":"39","author":[{"given":"Sophie","family":"Hodin","sequence":"first","affiliation":[{"name":"Saint\u2010Etienne"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Escal","sequence":"additional","affiliation":[{"name":"Saint\u2010Etienne"},{"name":"Laboratoire de Pharmacologie \u2013 Toxicologie CHU de Saint\u2010Etienne  Saint\u2010Etienne France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Feliu","sequence":"additional","affiliation":[{"name":"UR3801 PPF, Universit\u00e9 de Reims Champagne Ardenne  Reims France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Pontis","sequence":"additional","affiliation":[{"name":"CRH, CRC\u2010MHC (Centre de R\u00e9f\u00e9rence de l'H\u00e9mophilie, Centre de Ressource et de Comp\u00e9tence des Maladies H\u00e9morragiques Constitutionnelles) University Hospital  Rennes 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France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7134-0713","authenticated-orcid":false,"given":"Xavier","family":"Delavenne","sequence":"additional","affiliation":[{"name":"Saint\u2010Etienne"},{"name":"Laboratoire de Pharmacologie \u2013 Toxicologie CHU de Saint\u2010Etienne  Saint\u2010Etienne 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