{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:35:48Z","timestamp":1790631348600,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,10,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Semen Hoveniae (SH), known as Zhijuzi in Chinese, is extensively utilized in China for the management of alcoholic liver disease (ALD) due to its recognized detoxification properties. Despite its extensive historical use, the detailed chemical profile and anti\u2010ALD mechanisms of SH remain inadequately understood, significantly restricting its further therapeutic development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The chemical constituents of SH were systematically profiled using ultra high\u2010performance liquid chromatography coupled with time\u2010of\u2010flight mass spectrometry (UHPLC\u2010Q\u2010TOF MS). Potential molecular targets of identified compounds were predicted using the SwissTargetPrediction platform. Common targets were subsequently analyzed via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis using the DAVID database. Network pharmacology results were validated by molecular docking.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Seventy\u2010six compounds were identified or tentatively characterized in SH, including 50 flavonoids, 15 saponins, 5 terpenes, 3 alkaloids, 2 phenylpropanoids, and 1 other type, among which seven were unambiguously identified using reference standards. Furthermore, seven potentially novel components were identified. Network pharmacology and molecular docking analyses elucidated the molecular mechanisms underlying SH's therapeutic effects on ALD. Three core molecular targets\u2014AKT1, SRC, and EGFR\u2014were identified. Key pathways closely related to ALD, such as glutathione metabolism and arachidonic acid metabolism, were notably enriched, suggesting their crucial roles in SH's hepatoprotective mechanisms. Molecular docking studies confirmed strong binding affinities (binding energies lower than \u22125.0 kcal\/mol) between six active compounds (laricetrin, apigenin, quercetin, kaempferol, myricetin, and syringetin) and the three core targets (AKT1, SRC, and EGFR).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study comprehensively characterizes the chemical compositions of SH and elucidates its potential mechanisms against ALD. These findings substantiate the hepatoprotective potential of SH, providing a solid scientific foundation for its traditional use and promoting the development of novel therapeutic approaches for ALD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10097","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T03:15:38Z","timestamp":1751339738000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Phytochemical Analysis of Semen Hoveniae and Its Potential Mechanism Against Alcoholic Liver Disease via an Integrated Approach Combining UHPLC\u2010Q\u2010TOF MS, Network Pharmacology, and Molecular Docking"],"prefix":"10.1002","volume":"39","author":[{"given":"Min","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Emergency and Trauma of Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital Hainan Medical University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuo\u2010chun","family":"Wei","sequence":"additional","affiliation":[{"name":"Binhaiwan Central Hospital of Dongguan  Dongguan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1255-4222","authenticated-orcid":false,"given":"Feng\u2010xiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences Guangxi Normal University  Guilin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-7518-9553","authenticated-orcid":false,"given":"Hai\u2010jun","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Emergency and Trauma of Ministry of Education, Key Laboratory of Hainan Trauma and Disaster Rescue, The First Affiliated Hospital Hainan Medical University  Haikou China"},{"name":"General Medicine and Continuous Education College Hainan Medical University  Haikou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.35"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.09.002"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1337633"},{"key":"e_1_2_9_5_1","doi-asserted-by":"crossref","unstructured":"G.Sferrazza G.Brusotti M.Zonfrillo et\u00a0al. \u201cHovenia dulcisThumberg: Phytochemistry Pharmacology Toxicology and Regulatory Framework for Its Use in the European Union \u201d26 no.4(2021):903.","DOI":"10.3390\/molecules26040903"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100656"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116628"},{"key":"e_1_2_9_8_1","unstructured":"J.Hai\u2010yang \u201cStudy on Chemical Constituents From the Seeds ofHovenia acerbaLindl. Jilin Province China \u201d College of Chemistry Jilin University (2015)."},{"issue":"3","key":"e_1_2_9_9_1","first-page":"143","article-title":"Chemical Compositions of the Genus Hovenia","volume":"9","author":"Bao\u2010Jun X.","year":"2003","journal-title":"Natural Product Sciences"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bse.2020.104221"},{"issue":"3","key":"e_1_2_9_11_1","first-page":"575","article-title":"Research Progress on the Chemical Components and Pharmacological Activities of Hovenia dulcis","volume":"27","author":"Ji A. L. 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