{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:50:27Z","timestamp":1772981427255,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,11,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Alpha\u2010hemoglobin stabilizing protein (AHSP) is an erythroid\u2010specific protein forming a stable complex with free \u03b1\u2010hemoglobin, but not with \u03b2\u2010hemoglobin or hemoglobin A (\u03b1\n                      <jats:sub>2<\/jats:sub>\n                      \u03b2\n                      <jats:sub>2<\/jats:sub>\n                      ), thus preventing harmful aggregation of \u03b1\u2010hemoglobin during normal erythroid cell development and avoiding its pro\u2010oxidant activity. Although its function has been extensively studied in erythroid cells, its presence in preterm newborns' oral fluid remains unexplored. Given the high susceptibility of preterm infants to hematological disorders, characterizing AHSP in their oral fluid could provide valuable insights into fetal erythropoiesis and its potential role as a biomarker for neonatal anemia and transfusion needs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The primary structure of AHSP was determined by high\u2010resolution top\u2010down proteomic analysis using a nano\u2010HPLC\u2013ESI\u2013MS\/MS approach on oral fluid samples from preterm newborns. Specimens were collected non\u2010invasively from infants, promptly treated with formic acid, and analyzed with an Orbitrap\u2010based mass spectrometry platform. The intact protein's molecular mass and fragmentation pattern were assessed to determine its primary structure and post\u2010translational modifications. Statistical analysis was performed to explore correlations between AHSP presence and neonatal clinical parameters, including anemia and transfusion needs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The experimental monoisotopic molecular mass value [M\u2009+\u2009H\n                      <jats:sup>+<\/jats:sup>\n                      ]\n                      <jats:sup>1+<\/jats:sup>\n                      at\n                      <jats:italic>m\/z<\/jats:italic>\n                      11744.958\u2009\u00b1\u20090.3 was inconsistent with the protein sequence reported in the literature, and the MS\/MS fragmentation pattern was in agreement with the loss of the N\u2010terminal methionine residue followed by N\u03b1\u2010terminal acetylation, a very common post\u2010translational modification, now recognized as having an important role in modulating protein function, localization and protein stability and turnover. The sporadic samples having detectable amounts of AHSP resulted from preterm newborns with severe anemic status, while all were submitted to iron supplementation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Although the data obtained so far cannot be used for quantitative analysis or statistical evaluation, AHSP appears to stand out as a potential early biomarker of neonatal hematological disorders, highlighting a new perspective for future investigations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10107","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T06:59:22Z","timestamp":1752821962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Characterization of N\u2010Terminal Acetylated \u03b1\u2010Hemoglobin Stabilizing Protein (AHSP) by Top\u2010Down High\u2010Resolution Mass Spectrometry From Human Preterm Newborns Oral Fluid"],"prefix":"10.1002","volume":"39","author":[{"given":"Federica","family":"Iavarone","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore  Rome Italy"},{"name":"Fondazione Policlinico Universitario \u201cA. Gemelli\u201d\u2014IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Tirone","sequence":"additional","affiliation":[{"name":"Neonatology Unit, Department of Woman and Child Health and Public Health Fondazione Policlinico Universitario \u201cAgostino Gemelli,\u201d IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Fattore","sequence":"additional","affiliation":[{"name":"Neonatology Unit, Department of Woman and Child Health and Public Health Fondazione Policlinico Universitario \u201cAgostino Gemelli,\u201d IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"De\u00a0Tomaso","sequence":"additional","affiliation":[{"name":"Neonatology Unit, Department of Woman and Child Health and Public Health Fondazione Policlinico Universitario \u201cAgostino Gemelli,\u201d IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoletta","family":"Menzella","sequence":"additional","affiliation":[{"name":"Neonatology Unit, Department of Woman and Child Health and Public Health Fondazione Policlinico Universitario \u201cAgostino Gemelli,\u201d IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Vento","sequence":"additional","affiliation":[{"name":"Neonatology Unit, Department of Woman and Child Health and Public Health Fondazione Policlinico Universitario \u201cAgostino Gemelli,\u201d IRCCS  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Olianas","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Della Vita e Dell'ambiente, Sezione Biomedica Universit\u00e0 di Cagliari  Monserrato Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Manconi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Della Vita e Dell'ambiente, Sezione Biomedica Universit\u00e0 di Cagliari  Monserrato Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiziana","family":"Cabras","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Della Vita e Dell'ambiente, Sezione Biomedica Universit\u00e0 di Cagliari  Monserrato Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Guadalupi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Della Vita e Dell'ambiente, Sezione Biomedica Universit\u00e0 di Cagliari  Monserrato Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0903-4675","authenticated-orcid":false,"given":"Cristina","family":"Contini","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Della Vita e Dell'ambiente, Sezione Biomedica Universit\u00e0 di Cagliari  Monserrato Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mozhgan","family":"Boroumand","sequence":"additional","affiliation":[{"name":"National Institute on Aging, NIH  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Desiderio","sequence":"additional","affiliation":[{"name":"Istituto di Scienze e Tecnologie Chimiche \u201cGiulio Natta,\u201d Consiglio Nazionale Delle Ricerche  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Muntiu","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie Universit\u00e0 Cattolica del Sacro Cuore  Rome Italy"},{"name":"Fondazione Policlinico Universitario \u201cA. 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