{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:09Z","timestamp":1772118729909,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,8,26]],"date-time":"2025-08-26T00:00:00Z","timestamp":1756166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Shiduqing Capsule, a clinically widely used Chinese patent medicine, is indicated for skin itching, dryness, and desquamation caused by blood deficiency with wind\u2010dryness syndrome. However, the tissue distribution characteristics of its active components in\u00a0vivo and its pharmacological mechanisms in treating eczema remain unclear. This study aims to identify the bioactive components of Shiduqing Capsule, characterize their in\u00a0vivo tissue distribution patterns, and subsequently elucidate the underlying therapeutic mechanisms against eczema based on these findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study employed high\u2010resolution mass spectrometry (HRMS) coupled with the Global Natural Products Social Molecular Networking (GNPS) platform to comprehensively identify the chemical constituents of Shiduqing Capsule in\u00a0vivo, while simultaneously characterizing their tissue\u2010specific distribution profiles. Furthermore, network pharmacology approaches were utilized to systematically investigate the therapeutic mechanisms underlying Shiduqing Capsule's efficacy against eczema.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Through systematic chemical composition analysis, 51 blood\u2010absorbed components were identified in Shiduqing Capsule using advanced analytical techniques. Further GNPS\u2010assisted mass spectrometry analysis revealed its predominant composition of flavonoids, lipids, terpenoids, and phenylpropanoids. Network pharmacology investigation demonstrated that the capsule's anti\u2010eczema effects are mediated through critical pathways including TNF signaling and Th17 cell differentiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study systematically analyzed the in\u00a0vivo components of Shiduqing Capsule and their tissue distribution characteristics, while elucidating the underlying mechanisms of its therapeutic effects against eczema.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10128","type":"journal-article","created":{"date-parts":[[2025,8,27]],"date-time":"2025-08-27T04:41:16Z","timestamp":1756269676000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of In\u00a0Vivo Components in Shiduqing Capsule Using High\u2010Resolution Mass Spectrometry and Network Pharmacology\u2010Based Analysis of Its Therapeutic Mechanisms Against Eczema"],"prefix":"10.1002","volume":"39","author":[{"given":"Shuang","family":"Li","sequence":"first","affiliation":[{"name":"Department of Child Health Care Xiangyang No. 1 People's Hospital, Hubei University of Medicine  Xiangyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Child Health Care Xiangyang No. 1 People's Hospital, Hubei University of Medicine  Xiangyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Postgraduate Union Training Base of Xiangyang No. 1 People's Hospital, School of Medicine Wuhan University of Science and Technology  Xiangyang Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayiguzhali","family":"Yusun","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Postgraduate Union Training Base of Xiangyang No. 1 People's Hospital, School of Medicine Wuhan University of Science and Technology  Xiangyang Hubei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mo","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Georgia Institute of Technology  Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun","family":"Bao","sequence":"additional","affiliation":[{"name":"Department of Child Health Care Xiangyang No. 1 People's Hospital, Hubei University of Medicine  Xiangyang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3649-2428","authenticated-orcid":false,"given":"Chenning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Child Health Care Xiangyang No. 1 People's Hospital, Hubei University of Medicine  Xiangyang China"},{"name":"National Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture Jiangsu Kanion Pharmaceutical Co., Ltd.  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