{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:36:00Z","timestamp":1780760160798,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T00:00:00Z","timestamp":1759104000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC2503100"],"award-info":[{"award-number":["2022YFC2503100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,12,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Kashin\u2013Beck disease (KBD) is an osteoarthropathy with no specific drug treatment. This study seeks to uncover the molecular mechanisms of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) in treating KBD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Integrated transcriptomic and metabolomic analyses were performed on peripheral blood samples collected from four KBD patients before and 1\u2009month after treatment. Differential gene expression was assessed using the DESeq package, while differential metabolites were identified through univariate and multivariate statistical analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After treatment, 5671 differential genes (|log\n                      <jats:sub>2<\/jats:sub>\n                      FC|\u2009\u2265\u20091, q\u2009&lt;\u20090.05) were identified, with 4954 upregulated and 717 downregulated. Enriched pathways included PI3K\u2010Akt, MAPK, ECM\u2010receptor interaction, Rap1, and Ras signaling. Untargeted metabolomics analyses revealed five differentially expressed metabolites (VIP\u2009\u2265\u20091, |log\n                      <jats:sub>2<\/jats:sub>\n                      FC|\u2009&gt;\u20090.58,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05): L\u2010glutamine, 2\u2010methylpropan\u20102\u2010amine, epsilon\u2010caprolactam, phosphocholine, and 1\u2010palmitoyl\u20102\u2010linoleoyl\u2010sn\u2010glycero\u20103\u2010phosphocholine. Metabolite enrichment analysis suggested alterations in amino acid and fatty acid metabolism. Integrated analysis revealed key pathways such as alanine, aspartate, and glutamate metabolism and arginine biosynthesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The combination of ibuprofen, chondroitin sulfate, and vitamins with minerals tablets (21) may alleviate KBD through modulation of key genes (GDNF, BDNF, CCL2, VEGFA, and PPARG) and glutamine\u2010related metabolic pathways.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10130","type":"journal-article","created":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T10:50:16Z","timestamp":1759143016000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Transcriptomic and Metabolomic Mechanism of Ibuprofen, Chondroitin Sulfate, and Vitamins With Minerals Tablets (21) for Kashin\u2013Beck Disease Treatment"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5756-5585","authenticated-orcid":false,"given":"Jinfeng","family":"Wen","sequence":"first","affiliation":[{"name":"School of Public Health, Health Science Center Xi'an Jiaotong University, Key Laboratory of Environmental and Endemic Diseases of National Health Commission of the People's Republic of China  Xi'an Shaanxi China"},{"name":"Sichuan Provincial People's Hospital East Sichuan Hospital and Dazhou First People's Hospital  Dazhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingxing","family":"Deng","sequence":"additional","affiliation":[{"name":"School of Public Health, Health Science Center Xi'an Jiaotong University, Key Laboratory of Environmental and Endemic Diseases of National Health Commission of the People's Republic of China  Xi'an Shaanxi 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