{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:14:58Z","timestamp":1772118898581,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2025,9,15]],"date-time":"2025-09-15T00:00:00Z","timestamp":1757894400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["AI147314"],"award-info":[{"award-number":["AI147314"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006636","name":"University of Maryland, Baltimore County","doi-asserted-by":"publisher","award":["5T32AI162579\u201003"],"award-info":[{"award-number":["5T32AI162579\u201003"]}],"id":[{"id":"10.13039\/100006636","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2025,12,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Ventriculitis is a life\u2010threatening infectious condition that requires rapid pathogen identification. Conventional diagnostic methods often require 24\u201348\u2009h of ex\u00a0vivo culture. The aim of this study was to evaluate a novel MALDI\u2010TOF MS approach for analyzing glycolipids for species\u2010level pathogen identification directly from cerebral spinal fluid (CSF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Pathogen identification was conducted with the fast lipid analysis technique (FLAT), combined with matrix\u2010assisted laser desorption ionization\u2010time of flight mass spectrometry (MALDI\u2010TOF MS). In this pilot study, 12 CSF samples were analyzed, comprising six culture\u2010positive and six true negative specimens. The FLAT method was applied to 1\u2009mL of CSF from each sample, enabling pathogen identification within approximately 1.5\u2009h.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>FLAT was performed directly on CSF in under 2\u2009h. Successful genus\u2010level identification for all six culture\u2010positive samples was achieved, with five out of six correctly identified at the species level. Importantly, culture\u2010negative samples did not produce any pathogen\u2010associated glycolipid fingerprints, indicating the method's specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study highlights the potential of FLAT followed by MALDI\u2010TOF MS as a valuable tool for expediting ventriculitis pathogen identification. By bypassing the need for culture and delivering results in about an hour, this approach could significantly reduce turnaround times and potentially improve patient outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10138","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T01:04:11Z","timestamp":1757984651000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Pilot Study Using Microbial Glycolipid Signatures to Diagnose Nosocomial Ventriculitis"],"prefix":"10.1002","volume":"39","author":[{"given":"Ian\u00a0P.","family":"O'Keefe","sequence":"first","affiliation":[{"name":"Department of Microbial Pathogenesis, School of Dentistry University of Maryland \u2013 Baltimore  Baltimore Maryland USA"},{"name":"Department of Biochemistry and Molecular Biology, School of Medicine University of Maryland  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Putnam","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine University of Maryland  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert\u00a0K.","family":"Ernst","sequence":"additional","affiliation":[{"name":"Department of Microbial Pathogenesis, School of Dentistry University of Maryland \u2013 Baltimore  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4977-0968","authenticated-orcid":false,"given":"James\u00a0B.","family":"Doub","sequence":"additional","affiliation":[{"name":"The Doub Laboratory of Translational Bacterial Research, School of Medicine University of Maryland  Baltimore Maryland USA"},{"name":"Division of Clinical Care and Research, Institute of Human Virology, School of Medicine University of Maryland  Baltimore Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2021.1866544"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/ofid\/ofab216"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2023.04.389"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw861"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3068"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jfda.2019.01.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00072\u201012"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941\u2010020\u201000403\u2010w"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03730\u201022"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010020\u201078401\u20103"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.00014\u201024"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3390\/diseases10040110"},{"issue":"3","key":"e_1_2_8_14_1","first-page":"246","article-title":"Diagn\u00f3stico de Meningitis\/Encefalitis en UCI con Sistema de PCR M\u00faltiple. \u00bfEs Tiempo de Cambio? [Meningitis\/Encephalitis Diagnosis in ICU Using Multiplex PCR System: Is It Time of Change?]","volume":"32","author":"L\u00f3pez\u2010Amor L.","year":"2019","journal-title":"Revista Espa\u00f1ola de Quimioterapia"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.596175"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.10138","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,25]],"date-time":"2025-11-25T14:52:00Z","timestamp":1764082320000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.10138"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,15]]},"references-count":14,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2025,12,30]]}},"alternative-id":["10.1002\/rcm.10138"],"URL":"https:\/\/doi.org\/10.1002\/rcm.10138","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.10138\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10138\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10138\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10138\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.10138\/v1\/review1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,15]]},"assertion":[{"value":"2025-05-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10138"}}