{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T10:10:45Z","timestamp":1765188645290,"version":"3.46.0"},"reference-count":72,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:00:00Z","timestamp":1761609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,1,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      The stable hydrogen isotope composition (\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      H) of plant compounds can serve as environmental or metabolic proxies, but interpretations are hindered by insufficient mechanistic understanding. This can be improved by analyzing the\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      H values of metabolic intermediates, such as sucrose, which is the direct substrate of cellulose and a main transport sugar. However, current preparation methods for carbohydrates in general and sucrose in particular are not time\u2010efficient.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We evaluated methods that use acetylation of soluble carbohydrate plant extracts to aid in purification as well as isotopic analysis of plant sugars such as sucrose. Extracts were obtained using either hot water or hot 80% ethanol. Acetylated extracts were then purified using an established liquid\u2013liquid separation (LL) method or a new solid phase extraction (SPE) method that we developed. We also evaluated glucose produced from starch after enzymatic digestion. Method performance was evaluated based on quantified yields and the impact on measured\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      H values.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Acetylated sucrose and starch\u2010derived glucose were sufficiently resolved for gas chromatography in all cases. No isotopic biasing was detected for any method. Acetylated sucrose yields differed among methods, with 80% ethanol resulting in approximately threefold higher extraction yield compared to water, and SPE giving smaller but still sufficient yields compared to LL. Sample throughput was doubled with the SPE method compared to the LL method, which allows for larger batch sizes compared to LL.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      We developed an efficient method to analyze compound\u2010specific plant carbohydrate\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      H values using gas chromatography\u2010isotope ratio mass spectrometry (GC\u2010IRMS). This can be applied in experiments aimed at investigating the processes that shape cellulose\n                      <jats:italic>\u03b4<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      H values, including deconvoluting the metabolic and hydro\u2010climatic sources of isotopic variation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.10161","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:58:33Z","timestamp":1761663513000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Efficient Solid Phase Extraction Method for Purification and Analysis of Compound\u2010Specific Plant Sugar Stable Hydrogen Isotope Values"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-0976-5573","authenticated-orcid":false,"given":"Selina","family":"Hugger","sequence":"first","affiliation":[{"name":"Department of Environmental Sciences \u2013 Botany University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meisha","family":"Holloway\u2010Phillips","sequence":"additional","affiliation":[{"name":"Department of Environmental Sciences \u2013 Botany University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ansgar","family":"Kahmen","sequence":"additional","affiliation":[{"name":"Department of Environmental Sciences \u2013 Botany University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2716-7770","authenticated-orcid":false,"given":"Daniel\u00a0B.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Environmental Sciences \u2013 Botany University of Basel  Basel Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,10,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1130\/0091\u20107613(2003)031<0463:HEFTME>2.0.CO;2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18113"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.93.1.325"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016\u20107037(99)00195\u20107"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpad014"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20103040.1999.00440.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adl3591"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14193"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1504493112"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18014"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15016"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.19248"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.20113"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17957"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18226"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dendro.2020.125788"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14362"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2022.113563"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemgeo.2008.01.011"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469\u20108137.2006.01843.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.00238"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erl134"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.5586\/asbp.3583"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.20057"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00192a021"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1029\/GM078p0367"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/1025601031000096781"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7118"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.10091"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/15.6.196"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b02475"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7872"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126413"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41538\u2010021\u201000092\u20105"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.8854"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.013078"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00035008"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600\u20100706.2002.980301.x"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/treephys\/tpy118"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2008.6.230"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097\u20100010(199811)78:3<435::AID\u2010JSFA139>3.0.CO;2\u2010Q"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2008.05.025"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/plb.70006"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/eco.1892"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00085"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5129"},{"key":"e_1_2_9_48_1","unstructured":"R Core Team R: A Language and Environment for Statistical Computing(2024) https:\/\/www.r\u2010project.org\/."},{"key":"e_1_2_9_49_1","unstructured":"Posit team RStudio: Integrated Development Environment for R(2024) http:\/\/www.posit.co\/."},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24277-4"},{"key":"e_1_2_9_51_1","unstructured":"H.WickhamandJ.Bryan readxl: Read Excel Files(2019) https:\/\/cran.r\u2010project.org\/package=readxl."},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v040.i01"},{"key":"e_1_2_9_53_1","unstructured":"H.Wickham R.Fran\u00e7ois L.Henry andK.M\u00fcller dplyr: A Grammar of Data Manipulation(2021) https:\/\/cran.r\u2010project.org\/package=dplyr."},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v021.i12"},{"key":"e_1_2_9_55_1","unstructured":"H.Wickham D.Vaughan andM.Girlichtidyr: Tidy Messy Data2024https:\/\/tidyr.tidyverse.org."},{"volume-title":"An {R} Companion to Applied Regression","year":"2019","author":"Fox J.","key":"e_1_2_9_56_1"},{"key":"e_1_2_9_57_1","unstructured":"H.Wickham forcats: Tools for Working With Categorical Variables (Factors)(2023) https:\/\/forcats.tidyverse.org\/."},{"key":"e_1_2_9_58_1","unstructured":"B.Auguie gridExtra: Miscellaneous Functions for \u201cGrid\u201d Graphics(2017) https:\/\/cran.r\u2010project.org\/package=gridExtra."},{"key":"e_1_2_9_59_1","unstructured":"A.Kassambara ggpubr: \u201cggplot2\u201d Based Publication Ready Plots(2020) https:\/\/cran.r\u2010project.org\/package=ggpubr."},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7431"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.88.4.1418"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1993.0011183X003300060037x"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14655"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1071\/pp01072"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.353"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4088"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf00076a041"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orggeochem.2013.09.005"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1039\/c5ay01052g"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orggeochem.2011.09.004"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20103040.1999.00420.x"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.6334"},{"key":"e_1_2_9_73_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18227"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.10161","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T10:07:47Z","timestamp":1765188467000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.10161"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,28]]},"references-count":72,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,1,30]]}},"alternative-id":["10.1002\/rcm.10161"],"URL":"https:\/\/doi.org\/10.1002\/rcm.10161","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2025,10,28]]},"assertion":[{"value":"2025-05-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e10161"}}