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On the other hand, although flavonoid glycosides frequently occur in the leaves of the Annonaceae family, some species, such as\n Onychopetalum periquino<\/jats:italic>\n , remain understudied on this topic.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The aqueous extract from the leaves of\n O. periquino<\/jats:italic>\n was filtered through celite and eluted with methanol. The methanol fraction was analyzed by DI\u2010MS in data\u2010dependent acquisition (DDA) mode with neutral loss scans for flavonoid glycosides recognition. Finally, a set of presumable flavonoid glycosides isomers was analyzed by LC\u2013MS\/MS and dereplicated through manual interpretation of MS\/MS spectra.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n DI\u2010MS analysis revealed the presence of flavonoid glycoside isomers, primarily derived from apigenin, luteolin, and quercetin, which were duly dereplicated through LC\u2013MS\/MS analysis as follows: vitexin (\n 1<\/jats:bold>\n ), isovitexin (\n 2<\/jats:bold>\n ), apigenin 7\u2010\n O<\/jats:italic>\n \u2010glucoside (\n 3<\/jats:bold>\n ), luteolin 6\u2010\n C<\/jats:italic>\n \u2010glucoside (\n 4<\/jats:bold>\n ), luteolin 8\u2010\n C<\/jats:italic>\n \u2010glucoside (\n 5<\/jats:bold>\n ), luteolin 7\u2010\n O<\/jats:italic>\n \u2010glucoside (\n 6<\/jats:bold>\n ), isoscoparin (\n 7<\/jats:bold>\n ), chrysoeriol 7\u2010\n O<\/jats:italic>\n \u2010glucoside (\n 8<\/jats:bold>\n ), diosmetin 7\u2010\n O<\/jats:italic>\n \u2010glucoside (\n 9<\/jats:bold>\n ), apigenin 8\u2010\n C<\/jats:italic>\n \u2010neohesperidoside (\n 10<\/jats:bold>\n ), apigenin 6\u2010\n C<\/jats:italic>\n \u2010neohesperidoside (\n 11<\/jats:bold>\n ), apigenin 7\u2010\n O<\/jats:italic>\n \u2010rutinoside (\n 12<\/jats:bold>\n ), quercetin 3\u2010\n O<\/jats:italic>\n \u2010glucoside\u20107\u2010\n O<\/jats:italic>\n \u2010rhamnoside (\n 13<\/jats:bold>\n ), and quercetin 3\u2010\n O<\/jats:italic>\n \u2010rutinoside (\n 14<\/jats:bold>\n ).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n This study demonstrated the efficacy of the proposed strategy in identifying and differentiating flavonoid glycoside isomers in\n O. periquino<\/jats:italic>\n leaves. Therefore, this workflow is an effective approach for the comprehensive characterization of flavonoid glycoside isomers in future dereplication works. Additionally, it showed how structural differences, such as the type of linkage and position of sugars, influence their fragmentation patterns.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.10163","type":"journal-article","created":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T14:56:22Z","timestamp":1761663382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrative Approach Based on DI\u2010MS and LC\u2013MS\/MS Analysis for Comprehensive Characterization of Flavonoid Glycoside Isomers From\n Onychopetalum periquino<\/i>\n (Annonaceae)"],"prefix":"10.1002","volume":"40","author":[{"given":"Ana\u00a0Paula\u00a0A.","family":"Castro","sequence":"first","affiliation":[{"name":"Programa de P\u00f3s\u2010Gradua\u00e7\u00e3o em Qu\u00edmica Universidade Federal Do Amazonas Manaus AM Brazil"},{"name":"Centro de Apoio Multidisciplinar Universidade Federal Do Amazonas Manaus AM 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