{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:09Z","timestamp":1772118729983,"version":"3.50.1"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,30]],"date-time":"2025-11-30T00:00:00Z","timestamp":1764460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,2,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Rhodiola crenulata<\/jats:italic>\n                      (Hook. f. et Thoms.) H. Ohba (\n                      <jats:italic>R.\u2009crenulata<\/jats:italic>\n                      ) is widely acknowledged as a traditional medicine in both Asia and Europe, renowned for its exceptional health\u2010promoting properties. Currently, galloylated polyphenols are distinguished by their high structural similarity and notable isomerism, presenting considerable challenges in their annotation and evaluation. However, there is currently a lack of detailed information on the galloylated polyphenols of\n                      <jats:italic>R.\u2009crenulata<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We optimized the extraction conditions for galloylated polyphenols based on single\u2010factor and response surface experiments. The galloylated polyphenol components were enriched and isolated via HP\u201020 macroporous resin column chromatography. To this end, we proposed a comprehensive annotation and evaluation of the galloylated polyphenols in\n                      <jats:italic>R.\u2009crenulata<\/jats:italic>\n                      by utilizing an improved precursor ion list (PIL)\u2013data dependent acquisition (DDA)\/mass spectrometry (MS) combined with the biogenetic building block strategy, along with the preparation and evaluation of the refined fractions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      An acetone concentration of 65% and a solid\u2013liquid ratio of 1:29 were employed to extract galloylated polyphenols at 50\u00b0C for 46\u2009min, which facilitated the maximum extraction of these compounds. The galloylated polyphenols were characteristically enriched in a 30% ethanol elution using an HP\u201020 macroporous resin column chromatography. Subsequently, we annotated 56 galloylated polyphenols from\n                      <jats:italic>R.\u2009crenulata<\/jats:italic>\n                      , including 24 galloyl catechins, 17 gallotannins, 7 gallic acid esters, 4 galloyl salidrosides, 3 gallic acids, and 1 ellagitannin. These galloylated polyphenols and their fine fractions all showed significant nitric oxide inhibitory activity and exhibited potential anti\u2010inflammatory effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Based on this study, galloylated polyphenols are demonstrated to be a class of abundant and bioactive constituents in\n                      <jats:italic>R.\u2009crenulata<\/jats:italic>\n                      , thereby providing a foundation for further elucidating the material basis of its medicinal effects.\n                    <\/jats:p>\n                  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