{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:34:05Z","timestamp":1789202045231,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2003,7,16]],"date-time":"2003-07-16T00:00:00Z","timestamp":1058313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Commissariat \u00e0 l'Energie Atomique"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2003,8,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Metallochaperones are soluble proteins involved in metal transport and regulation\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Copper metallochaperones belong to a structural family of metal binding domains displaying a ferredoxin\u2010like fold (\n                    <jats:italic>\u03b2\u03b1\u03b2\u03b2\u03b1\u03b2<\/jats:italic>\n                    ) and a consensus metal\u2010binding motif MXCXXC. The metal\u2010binding selectivities for this class of proteins are poorly documented so far. The present study focuses on the measurement of the selectivity of the copper metallochaperone CopZ from\n                    <jats:italic>Enterococcus hirae<\/jats:italic>\n                    for different metal ions using an experimental approach based on electrospray ionization mass spectrometry (ESI\u2010MS). All the metal cations tested, i.e. Cu(I), Cu(II), Hg(II), Cd(II) and Co(II), form specific metal complexes with CopZ. The study of a chemically modified CopZ as well as variants of CopZ in the active site demonstrated that the complexes observed by ESI\u2010MS, i.e. in the gas phase, corresponded to the complexes previously observed by other analytical methods in solution. Competition experiments allowed the classification of the metal ions by increasing affinities for CopZ as follows: Co\u2009\u226a\u2009Cd\u2009&lt;\u2009Hg\u2009&lt;\u2009Cu. A dissociation constant in the range of 20\u2009\u03bcM was determined for cobalt. The affinity of CopZ for the other metals tested was found to be higher, with dissociation constants smaller than micromolar. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1130","type":"journal-article","created":{"date-parts":[[2003,7,18]],"date-time":"2003-07-18T06:10:00Z","timestamp":1058508600000},"page":"1889-1896","source":"Crossref","is-referenced-by-count":34,"title":["Analysis of the metal\u2010binding selectivity of the metallochaperone CopZ from\n                    <i>Enterococcus hirae<\/i>\n                    by electrospray ionization mass spectrometry"],"prefix":"10.1002","volume":"17","author":[{"given":"Agathe","family":"Urvoas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badia","family":"Amekraz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Moulin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Le 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