{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:51:14Z","timestamp":1761511874171,"version":"build-2065373602"},"reference-count":11,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2003,10,23]],"date-time":"2003-10-23T00:00:00Z","timestamp":1066867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2003,11,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A generic high\u2010throughput liquid chromatography (HTLC) tandem mass spectrometry (MS\/MS) assay for the determination of compound I in human urine and dialysate (hemodialysis) was developed and validated. By using the HTLC on\u2010line extraction technique, sample pretreatment was not necessary. The sample was directly injected onto a narrow bore large particle size extraction column (50\u2009\u00d7\u20091.0\u2009mm, 60\u2009\u03bcm) where the sample matrix was rapidly washed away using a high flow rate (5\u2009mL\/min) aqueous mobile phase while analytes were retained. The analytes were subsequently eluted from the extraction column onto an analytical column using an organic\u2010enriched mobile phase prior to mass spectrometric detection. The analytes were then eluted from the analytical column to the mass spectrometer for the determination. The linear dynamic range was 2.0\u20136000\u2009ng\/mL for the urine assay and 0.1\u2013300\u2009ng\/mL for the dialysate assay. Intraday accuracy and precision were evaluated by analyzing five replicates of calibration standards at all concentrations used to construct the standard curve. For the urine assay, the precision (RSD%, n\u2009=\u20095) ranged from 1.9 to 8.0% and the accuracy ranged from 87.8 to 105.2% of nominal value. For the dialysate assay, the precision (RSD%, n\u2009=\u20095) ranged from 1.1 to 10.0% and the accuracy from 94.5 to 105.2% of nominal value. In\u2010source fragmentation of the acyl glucuronide metabolite (compound III) did not interfere with the determination of parent compound I. The developed HTLC\/MS\/MS methodology was specific for compound I in the presence of compound III. Column life\u2010time is increased and sample analysis time is decreased over traditional reversed\u2010phase methods when direct injection assays for urine and dialysate are coupled with the technology of HTLC. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/rcm.1221","type":"journal-article","created":{"date-parts":[[2003,11,4]],"date-time":"2003-11-04T03:00:46Z","timestamp":1067914846000},"page":"2475-2482","source":"Crossref","is-referenced-by-count":12,"title":["A direct injection high\u2010throughput liquid chromatography tandem mass spectrometry method for the determination of a new orally active\n                    <i>\u03b1<\/i>\n                    <sub>v<\/sub>\n                    <i>\u03b2<\/i>\n                    <sub>3<\/sub>\n                    antagonist in human urine and dialysate"],"prefix":"10.1002","volume":"17","author":[{"given":"Wei","family":"Zeng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy Qiu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alison L.","family":"Fisher","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald G.","family":"Musson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,23]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00232-1"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0731-7085(01)00406-X"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.669"},{"key":"e_1_2_5_5_2","unstructured":"QuinnHM TakarewskiJJ.International Patent. WO 97\/16724 1997."},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(199712)11:18<1953::AID-RCM102>3.0.CO;2-Z"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02491558"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00535-X"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0231(20001215)14:23<2282::AID-RCM164>3.0.CO;2-V"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(20000215)14:3<141::AID-RCM856>3.0.CO;2-I"},{"key":"e_1_2_5_11_2","unstructured":"Food and Drug Administration U.S. Department of Health and Human Services Guidance for Industry: Bioanalytical Method Validation May 2001."},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(98)00437-X"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.1221","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.1221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:09:56Z","timestamp":1761487796000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.1221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10,23]]},"references-count":11,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2003,11,30]]}},"alternative-id":["10.1002\/rcm.1221"],"URL":"https:\/\/doi.org\/10.1002\/rcm.1221","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2003,10,23]]}}}