{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:16:41Z","timestamp":1761488201313,"version":"build-2065373602"},"reference-count":6,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2005,4,8]],"date-time":"2005-04-08T00:00:00Z","timestamp":1112918400000},"content-version":"vor","delay-in-days":4146,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[1993,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Two congeners Q\n                    <jats:sub>4<\/jats:sub>\n                    and Q\n                    <jats:sub>5<\/jats:sub>\n                    inferred in earlier analyses to be cyclic succinimide\u2010type dehydration product; of recombinant hirudin (variant 1) were structurally fully characterized. After isotopic labeling by ring\u2010opening with H\n                    <jats:sub>2<\/jats:sub>\n                    <jats:sup>18<\/jats:sup>\n                    O, the suspected anhydro\u2010positions Asp\n                    <jats:sup>53<\/jats:sup>\n                    and Asp\n                    <jats:sup>33<\/jats:sup>\n                    were confirmed by tandem mass spectrometry (MS\/MS) sequencing of relevant smaller peplides directly in the enzymatic hydrolysates (V8 protease) using electrospray MS\/MS. The chosen strategy proved highly efficient and sensitive.\n                  <\/jats:p>","DOI":"10.1002\/rcm.1290071205","type":"journal-article","created":{"date-parts":[[2005,5,29]],"date-time":"2005-05-29T05:51:39Z","timestamp":1117345899000},"page":"1082-1085","source":"Crossref","is-referenced-by-count":14,"title":["Characterization of succinimide\u2010type dehydration products of recombinant hirudin variant 1 by electrospray tandem mass spectrometry"],"prefix":"10.1002","volume":"7","author":[{"given":"H.","family":"Grossenbacher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"M\u00e4rki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Coulot","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"M\u00fcller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Richter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,4,8]]},"reference":[{"key":"e_1_2_1_2_2","unstructured":"W.M\u00e4rki H.Grossenbacher F.RaschdorfandM.Liersch 5th European Congress on Biotechnology Copenhagen July 8\u2010131990. Proceedings Vol. 2 p.945."},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2860(93)80092-A"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00150a024"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00053a042"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","unstructured":"S.Capasso L.Mazzarella F.Sica A.ZagariandS.Salvadori J. Chem. Soc. Chem. Commun.919(1992).","DOI":"10.1039\/c39920000919"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00024a003"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.1290071205","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.1290071205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:05:27Z","timestamp":1761487527000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.1290071205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12]]},"references-count":6,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1993,12]]}},"alternative-id":["10.1002\/rcm.1290071205"],"URL":"https:\/\/doi.org\/10.1002\/rcm.1290071205","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[1993,12]]}}}